Anti-Hu SIGLEC8 Purified

Anti-Hu SIGLEC8 Purified
Regulatory status
RUO
Antigen
SIGLEC8
Clone
7C9
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-846-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 7C9 recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed e.g. in lymph nodes and spleen.
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
SAF2
Antigen description
SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.
Entrez Gene ID 27181
UniProt ID Q9NYZ4
11-846_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human SIGLEC8 (7C9) purified antibody (concentration in sample 6 μg/ml; GAM APC).

Product specific references:

Galeotti C, Stephen-Victor E, Karnam A, Das M, Gilardin L, Maddur MS, Wymann S, Vonarburg C, Chevailler A, Dimitrov JD, Benveniste O, Bruhns P, Kaveri SV, Bayry J: Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE. J Allergy Clin Immunol. 2019 Aug;144(2):524-535.e8.
PubMed
Kano G, Bochner BS, Zimmermann N: Regulation of Siglec-8-induced intracellular reactive oxygen species production and eosinophil cell death by Src family kinases. Immunobiology. 2017 Feb;222(2):343-349.
PubMed
Arakawa S, Suzukawa M, Ohshima N, Tashimo H, Asari I, Matsui H, Kobayashi N, Shoji S, Nagase T, Ohta K: Expression of Siglec-8 is regulated by interleukin-5, and serum levels of soluble Siglec-8 may predict responsiveness of severe eosinophilic asthma to mepolizumab. Allergol Int. 2018 Sep;67S:S41-S44.
PubMed
Kano G, Almanan M, Bochner BS, Zimmermann N: Mechanism of Siglec-8-mediated cell death in IL-5-activated eosinophils: role for reactive oxygen species-enhanced MEK/ERK activation. J Allergy Clin Immunol. 2013 Aug;132(2):437-45.
PubMed
Variant
0.1 mg
11-846-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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