Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu SIGLEC8 Purified

Anti-Hu SIGLEC8 Purified

Regulatory status

RUO

Antigen

SIGLEC8

Clone

7C9

Format

Purified

Reactivity

Human

Application

ELISA, IP, FC (QC tested)

Variant

0.1 mg

11-846-C100

In stock

140.00 USD

Variant

0.1 mg

11-846-C100

In stock

140.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 7C9 recognizes an extracellular epitope of SIGLEC8, an eosinophil marker, expressed e.g. in lymph nodes and spleen.

Application

ELISA, IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SAF2

Antigen description

SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.

Entrez Gene ID 27181

UniProt ID Q9NYZ4

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human SIGLEC8 (7C9) purified antibody (concentration in sample 6 μg/ml; GAM APC).

Product specific references:

Galeotti C, Stephen-Victor E, Karnam A, Das M, Gilardin L, Maddur MS, Wymann S, Vonarburg C, Chevailler A, Dimitrov JD, Benveniste O, Bruhns P, Kaveri SV, Bayry J: Intravenous immunoglobulin induces IL-4 in human basophils by signaling through surface-bound IgE. J Allergy Clin Immunol. 2019 Aug;144(2):524-535.e8.
PubMed

Kano G, Bochner BS, Zimmermann N: Regulation of Siglec-8-induced intracellular reactive oxygen species production and eosinophil cell death by Src family kinases. Immunobiology. 2017 Feb;222(2):343-349.
PubMed

Arakawa S, Suzukawa M, Ohshima N, Tashimo H, Asari I, Matsui H, Kobayashi N, Shoji S, Nagase T, Ohta K: Expression of Siglec-8 is regulated by interleukin-5, and serum levels of soluble Siglec-8 may predict responsiveness of severe eosinophilic asthma to mepolizumab. Allergol Int. 2018 Sep;67S:S41-S44.
PubMed

Kano G, Almanan M, Bochner BS, Zimmermann N: Mechanism of Siglec-8-mediated cell death in IL-5-activated eosinophils: role for reactive oxygen species-enhanced MEK/ERK activation. J Allergy Clin Immunol. 2013 Aug;132(2):437-45.
PubMed