Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu SIGLEC10 PE

Anti-Hu SIGLEC10 PE

Regulatory status

RUO

Antigen

SIGLEC10

Clone

5G6

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-932-T100

In stock

242.00 USD

Variant

100 tests

1P-932-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 5G6 recognizes an extracellular epitope of human SIGLEC10, a sialic acid-binding lectin expressed on subsets of human leucocytes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

SIGLEC10 extracellular domain fused with human IgG1 Fc fragment

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PRO940, SGL2

Antigen description

SIGLEC10 is a CD33-related receptor of sialoglycans, expressed on eosinophils, monocytes, a subpopulation of NK cells, and at lower level on B cells. Its murine ortholog is Siglec G. SIGLEC10 seems to act as an immunomodulatory receptor, which binds to VAP-1, a glycoprotein expressed on endothelium under inflammatory conditions. Another ligand of SIGLEC10 is CD24, a marker of poorer prognosis in carcinomas.

Entrez Gene ID 89790

UniProt ID Q96LC7

Anti-SIGLEC10 PE antibody (clone 5G6) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Mouse monoclonal anti-human SIGLEC10 PE antibody (clone 5G6) was used in amount of 10 µl in 100 µl of blood sample (2 x 10⁶ cells).

Expression profiling on peripheral blood subsets using Anti-SIGLEC10 PE antibody (clone 5G6).

HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes.
Suspension of blood leukocytes isolated from buffy coats (2 x 10⁶ cells) was added to the mixture of anti-human SIGLEC10 PE antibody (clone 5G6, 10 μl reagent / 100 μl of stained blood sample) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min. Next, optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) were added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution (#ED7066) and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Product specific references:

Stephenson HN, Mills DC, Jones H, Milioris E, Copland A, Dorrell N, Wren BW, Crocker PR, Escors D, Bajaj-Elliott M: Pseudaminic acid on Campylobacter jejuni flagella modulates dendritic cell IL-10 expression via Siglec-10 receptor: A novel flagellin-host interaction. J Infect Dis. 2014 Nov 1;210(9):1487-98.
PubMed

Nguyen DH, Ball ED, Varki A: Myeloid precursors and acute myeloid leukemia cells express multiple CD33-related Siglecs. Exp Hematol. 2006 Jun;34(6):728-35.
PubMed

Läubli H, Kawanishi K, Vazhappilly CG, Matar R, Merheb M, Siddiqui SS: Tools to study and target the Siglec-sialic acid axis in cancer. FEBS J. 2021 Nov;288(21):6206-6225.
PubMed

Bhamidipati K, Silberstein JL, Chaichian Y, Baker MC, Lanz TV, Zia A, Rasheed YS, Cochran JR, Robinson WH: CD52 is elevated on B cells of SLE patients and regulates B cell function. Front Immunol. 2021 Feb 4;11:626820.
PubMed

Munday J, Kerr S, Ni J, Cornish AL, Zhang JQ, Nicoll G, Floyd H, Mattei MG, Moore P, Liu D, Crocker PR: Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor. Biochem J. 2001 Apr 15;355(Pt 2):489-97.
PubMed