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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu PODXL Alexa Fluor<sup>®</sup> 647
Anti-Hu PODXL Alexa Fluor
®
647
Regulatory status
RUO
Antigen
PODXL
Clone
PcMab-47
Format
Alexa Fluor
®
647
Reactivity
Human
Application
IHC, WB, FC (QC tested)
Excitation laser
red (633 nm)
Variant
0.1 mg
A6-899-C100
In stock
242.00 USD
Variant
0.1 mg
A6-899-C100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody PcMab-47 recognizes a glycosylation-dependent epitope (aa 207-210) on human PODXL, a highly glycosylated transmembrane glycoprotein expressed above all in many types of cancer tissues, and on embryonic stem cells.
Application
IHC, WB, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Human
Immunogen
recombinant PODXL ectodomain
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor
®
647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
PCLP-1, Gp200, podocalyxin like
Antigen description
PODXL is a highly glycosylated sialomucin, which is expressed in many types of tumors, as well as it is a well known marker of embryonic stem cells. Overexpression of PODXL is an independent predictor of cancer progression, metastasis, and poor outcome. PODXL promotes tumor growts and invasiveness, and is a potential target for antibody therapy.
Entrez Gene ID
5420
UniProt ID
O00592
Separation of HeLa cells stained using anti-human PODXL (PcMab-47) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml, same as PODXL Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (surface staining).
Product specific references:
Schulten HJ, Hussein D: Array expression meta-analysis of cancer stem cell genes identifies upregulation of PODXL especially in DCC low expression meningiomas. PLoS One 2019 May 13;14(5):e0215452.
PubMed
Itai S, Yamada S, Kaneko MK, Kato Y: Determination of critical epitope of PcMab-47 against human podocalyxin. Biochem Biophys Rep. 2018 Apr 24;14:78-82.
PubMed
Ogasawara S, Kaneko MK, Yamada S, Honma R, Nakamura T, Saidoh N, Yanaka M, Yoshida K, Fujii Y, Kato Y: PcMab-47: Novel antihuman podocalyxin monoclonal antibody for immunohistochemistry. Monoclon Antib Immunodiagn Immunother. 2017 Apr;36(2):50-56.
PubMed
Itai S, Ohishi T, Kaneko MK, Yamada S, Abe S, Nakamura T, Yanaka M, Chang YW, Ohba SI, Nishioka Y, Kawada M, Harada H, Kato Y: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget 2018 Apr 27;9(32):22480-22497.
PubMed
Kaneko MK, Ohishi T, Kawada M, Kato Y: A cancer-specific anti-podocalyxin monoclonal antibody (60-mG 2a-f) exerts antitumor effects in mouse xenograft models of pancreatic carcinoma. Biochem Biophys Rep. 2020 Oct 10;24:100826.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
A6-899-C100
In stock
242.00 USD
Variant
0.1 mg
A6-899-C100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
A6-632-C100
Mouse IgG1 Isotype Control Alexa Fluor
®
647
-134%
Clone
MOPC-21
Reg. status
RUO
Application
FC, FC-IC, WB, IHC-F, IHC-P, ICC
0.1 mg
231.00 USD
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