Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu NKp80 PE

Anti-Hu NKp80 PE

Regulatory status

RUO

Antigen

NKp80

Clone

5D12

Format

Reactivity

Human, Monkey

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-917-T100

In stock

242.00 USD

Variant

100 tests

1P-917-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 5D12 recognizes an extracellular epitope of human NKp80 (CLEC5C), a C-type lectin family member, expressed on NK cells and subsets of T cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Monkey

Negative species

Mouse, Rat

Immunogen

recombinant human NKp80 extracellular domain

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KLRF1, CLEC5C

Antigen description

NKp80, also known as CLEC5C or KLRF1, is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells.

Entrez Gene ID 51348

UniProt ID Q9NZS2

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human NKp80 (5D12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human NKp80 positive lymphocytes (red-filled) from NKp80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human NKp80 (5D12) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Dennehy KM, Klimosch SN, Steinle A: Cutting edge: NKp80 uses an atypical hemi-ITAM to trigger NK cytotoxicity. J Immunol. 2011 Jan 15;186(2):657-61.
PubMed

Campbell TM, McSharry BP, Steain M, Russell TA, Tscharke DC, Kennedy JJ, Slobedman B, Abendroth A: Functional paralysis of human natural killer cells by alphaherpesviruses. PLoS Pathog. 2019 Jun 13;15(6):e1007784
PubMed

Rückrich T, Steinle A: Attenuated natural killer (NK) cell activation through C-type lectin-like receptor NKp80 is due to an anomalous hemi-immunoreceptor tyrosine-based activation motif (HemITAM) with impaired Syk kinase recruitment capacity. J Biol Chem. 2013 Jun 14;288(24):17725-33.
PubMed