Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu Myeloperoxidase Purified

Anti-Hu Myeloperoxidase Purified

Regulatory status

RUO

Antigen

Myeloperoxidase

Clone

MPO421-8B2

Format

Purified

Reactivity

Human

Application

ELISA, FC (QC tested)

Variant

0.1 mg

11-791-C100

In stock

187.00 USD

0.025 mg

11-791-C025

Delivery 1 week

93.50 USD

Variant

0.1 mg

11-791-C100

In stock

187.00 USD

0.025 mg

11-791-C025

Delivery 1 week

93.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.

Application

ELISA, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Human myeloperoxidase

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MPO

Antigen description

Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.

Entrez Gene ID 4353

UniProt ID P05164

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 μg/ml) GAM APC.

General references:

Ximenes VF, Maghzal GJ, Turner R, Kato Y, Winterbourn CC, Kettle AJ: Serotonin as a physiological substrate for myeloperoxidase and its superoxide-dependent oxidation to cytotoxic tryptamine-4,5-dione. Biochem J. 2009 Dec 14;425(1):285-93.
PubMed

Hansson M, Olsson I, Nauseef WM: Biosynthesis, processing, and sorting of human myeloperoxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):214-24.
PubMed

Ximenes VF, Silva SO, Rodrigues MR, Catalani LH, Maghzal GJ, Kettle AJ, Campa A: Superoxide-dependent oxidation of melatonin by myeloperoxidase. J Biol Chem. 2005 Nov 18;280(46):38160-9.
PubMed

Chooklin S, Pereyaslov A, Bihalskyy I: Pathogenic role of myeloperoxidase in acute pancreatitis. Hepatobiliary Pancreat Dis Int. 2009 Dec;8(6):627-31.
PubMed

Murphy EJ, Maréchal A, Segal AW, Rich PR: CO binding and ligand discrimination in human myeloperoxidase. Biochemistry. 2010 Mar 16;49(10):2150-8.
PubMed

Product specific references:

Locke M, Francis RJ, Tsaousi E, Longstaff C: Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci Rep. 2020 Jul 16;10(1):11694.
PubMed