Anti-Hu Myeloperoxidase PE

Anti-Hu Myeloperoxidase PE
Regulatory status
RUO
Antigen
Myeloperoxidase
Clone
MPO421-8B2
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-791-T100
In stock
242.00 USD

25 tests
1P-791-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-791-T100
In stock
242.00 USD

25 tests
1P-791-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Human
Immunogen
Human myeloperoxidase
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
MPO
Antigen description
Myeloperoxidase (MPO) is a heme enzyme that is localized in azurophilic (primary) granules of myeloid cells and its synthesis occurs at an early stage of differentiation. The mature myeloperoxidase is a tetramer composed of two light (12 kDa) and two heavy (60 kDa) chains. This enzyme uses hydrogen peroxide to oxidize numerous substrates, including serotonin, melatonin or chloride, to produce reactive free radicals that contribute to immune reactions of myeloid cells against pathogens. Myeloperoxidase functions not only in host defense by mediating efficient microbial killing but also can contribute to progressive tissue damage in chronic inflammatory states such as atherosclerosis or acute pancreatitis.
Entrez Gene ID 4353
UniProt ID P05164
1P-791_FC_CDMaps_Profil značení
Anti-human Myeloperoxidase PE antibody (clone MPO421-8B2) works in works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm solution (#ED7397).
Mouse monoclonal anti-human Myeloperoxidase PE antibody (clone MPO421-8B2) was used in concentration 10 µg/ml in stained blood sample (2 x 106 cells).
1P-791_FC_CDMaps_Histogram
Expression profiling on peripheral blood subsets using Anti-human Myeloperoxidase PE antibody (clone MPO421-8B2).

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set (#ED7397).
HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for leukocyte isolation.
Suspension of blood leukocytes isolated from buffy coats (2 x 106 cells) was added to the mixture of optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min.
Next, EXCELLYSE XPerm buffer set (#ED7397) was used for leukocytes permeabilization according to the instruction procedure.
Mouse monoclonal anti-human Myeloperoxidase PE antibody (clone MPO421-8B2) was used in concentration 10 µg/ml in stained blood sample (2 x 106 cells).
1P-791_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-791_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

General references:

Ximenes VF, Maghzal GJ, Turner R, Kato Y, Winterbourn CC, Kettle AJ: Serotonin as a physiological substrate for myeloperoxidase and its superoxide-dependent oxidation to cytotoxic tryptamine-4,5-dione. Biochem J. 2009 Dec 14;425(1):285-93.
PubMed
Hansson M, Olsson I, Nauseef WM: Biosynthesis, processing, and sorting of human myeloperoxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):214-24.
PubMed
Ximenes VF, Silva SO, Rodrigues MR, Catalani LH, Maghzal GJ, Kettle AJ, Campa A: Superoxide-dependent oxidation of melatonin by myeloperoxidase. J Biol Chem. 2005 Nov 18;280(46):38160-9.
PubMed
Chooklin S, Pereyaslov A, Bihalskyy I: Pathogenic role of myeloperoxidase in acute pancreatitis. Hepatobiliary Pancreat Dis Int. 2009 Dec;8(6):627-31.
PubMed
Murphy EJ, Maréchal A, Segal AW, Rich PR: CO binding and ligand discrimination in human myeloperoxidase. Biochemistry. 2010 Mar 16;49(10):2150-8.
PubMed

Product specific references:

Locke M, Francis RJ, Tsaousi E, Longstaff C: Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci Rep. 2020 Jul 16;10(1):11694.
PubMed
Variant
100 tests
1P-791-T100
In stock
242.00 USD

25 tests
1P-791-T025
Delivery 1 week
121.00 USD
Variant
100 tests
1P-791-T100
In stock
242.00 USD

25 tests
1P-791-T025
Delivery 1 week
121.00 USD

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