Anti-Hu Myeloperoxidase FITC

Anti-Hu Myeloperoxidase FITC
Regulatory status
RUO
Antigen
Myeloperoxidase
Clone
MPO421-8B2
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-791-T100
In stock

25 tests
1F-791-T025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody MPO421-8B2 recognizes human myeloperoxidase, a heme protein present in intracellular granules of myeloblasts, neutrophils and monocytes. It is a marker of acute myelogenous leukemias and acute lymphoblastic leukemias.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human myeloperoxidase
Other names
MPO
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1F-791 FC
Flow cytometry analysis (intracellular staining) of human peripheral blood with anti-myeloperoxidase (MPO421-8B2) FITC.

General references:

Ximenes VF, Maghzal GJ, Turner R, Kato Y, Winterbourn CC, Kettle AJ: Serotonin as a physiological substrate for myeloperoxidase and its superoxide-dependent oxidation to cytotoxic tryptamine-4,5-dione. Biochem J. 2009 Dec 14;425(1):285-93.
PubMed
Hansson M, Olsson I, Nauseef WM: Biosynthesis, processing, and sorting of human myeloperoxidase. Arch Biochem Biophys. 2006 Jan 15;445(2):214-24.
PubMed
Ximenes VF, Silva SO, Rodrigues MR, Catalani LH, Maghzal GJ, Kettle AJ, Campa A: Superoxide-dependent oxidation of melatonin by myeloperoxidase. J Biol Chem. 2005 Nov 18;280(46):38160-9.
PubMed
Chooklin S, Pereyaslov A, Bihalskyy I: Pathogenic role of myeloperoxidase in acute pancreatitis. Hepatobiliary Pancreat Dis Int. 2009 Dec;8(6):627-31.
PubMed
Murphy EJ, Maréchal A, Segal AW, Rich PR: CO binding and ligand discrimination in human myeloperoxidase. Biochemistry. 2010 Mar 16;49(10):2150-8.
PubMed
Variant
100 tests
1F-791-T100
In stock

25 tests
1F-791-T025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock