Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu LOX1 APC

Anti-Hu LOX1 APC

Regulatory status

RUO

Antigen

LOX1

Clone

15C4

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-929-T100

In stock

242.00 USD

Variant

100 tests

1A-929-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 15C4 recognizes an extracellular epitope of LOX1, a C-type lectin transmembrane protein.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

fusion protein of human LOX1 ectodomain and of human IgG Fc

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

OLR1, SLOX1, SCARE1, CLEC8A

Antigen description

LOX1, a 31 kDa type II transmembrane protein, is a C-type lectin, functioning as a scavenger receptor for e.g. oxidized low density lipoprotein, apoptotic heat shock proteins, or CRP. It is expressed by macrophages, fibroblasts, platelets, endothelial cells, and smooth muscle cells, and its defects can lead to atherosclerosis. Its expression is enhanced under inflammatory conditions. Multiple splicing variants have been identified.

Entrez Gene ID 4973

UniProt ID P78380

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-LOX1 (15C4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-LOX1 (15C4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD11c (BU15) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of CD11c positive cells stained using anti-LOX1 (15C4) APC antibody (10 μl reagent / 100 μl of peripheral whole blood, red-filled) from CD11c positive cells stained using mouse IgG isotype control (MOPC-21) APC antibody (concentration in sample 1 μg/ml, same as LOX APC antibody concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

Product specific references:

Duluc D, Banchereau R, Gannevat J, Thompson-Snipes L, Blanck JP, Zurawski S, Zurawski G, Hong S, Urgell JR, Pascual V, Baldwin N, Stecher J, Carley M, Boreham M, Oh S: Transcriptional fingerprints of antigen-presenting cell subsets in the human vaginal mucosa and skin reflect tissue-specific immune microenvironments. Genome Med. 2014 Nov 25;6(11):98.
PubMed

Cassetta L, Bruderek K, Skrzeczynska J, Osiecka O, Hu X, Rundgren IM, Lin A, Santegoets K, Horzum U, Godinho-Santos A, Zelinsky G, Garcia-Tellez T, Bjelica S, Taciak B, Kittang AO, Höing B, Lang S, Dixon M, Müller V, Utíkal JS, Karakoç D, Yilmaz KB, Górka E, Bodnár L, Anastasiou OE, Bourgeois C, Baďura R, Kapinska-Mrowiecka M, Gotic M, Laan MT, Kers-Rebel E, Król M, Santibañez JF, Müller M, Dittmer U, de Sousa AE, Esendağli G, Adema G, Loré K, Ersvær E, Umansky V, Pollard JW, Cichy J, Brandau S: Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation. J Immunother Cancer. 2020 Sep;8(2):e001223.
PubMed

Joo H, Li D, Dullaers M, Kim TW, Duluc D, Upchurch K, Xue Y, Zurawski S, Le Grand R, Liu YJ, Kuroda M, Zurawski G, Oh S: C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses. Immunity 2014 Oct 16;41(4):592-604.
PubMed

Bayik D, Zhou Y, Park C, Hong C, Vail D, Silver DJ, Lauko A, Roversi G, Watson DC, Lo A, Alban TJ, McGraw M, Sorensen M, Grabowski MM, Otvos B, Vogelbaum MA, Horbinski C, Kristensen BW, Khalil AM, Hwang TH, Ahluwalia MS, Cheng F, Lathia JD: Myeloid-derived suppressor cell subsets drive glioblastoma growth in a sex-specific manner. Cancer Discov. 2020 Aug;10(8):1210-1225.
PubMed

Li D, Romain G, Flamar AL, Duluc D, Dullaers M, Li XH, Zurawski S, Bosquet N, Palucka AK, Le Grand R, O'Garra A, Zurawski G, Banchereau J, Oh S: Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells. J Exp Med. 2012 Jan 16;209(1):109-21.
PubMed