Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu LAR APC

Anti-Hu LAR APC

Regulatory status

RUO

Antigen

LAR

Clone

W7C6

Format

APC

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-830-C100

Ask for delivery term HERE

242.00 USD

Variant

0.1 mg

1A-830-C100

Ask for delivery term HERE

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody W7C6 recognizes an extracellular epitope of protein tyrosine phosphatase LAR, a marker of mesenchymal stem cells.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Non-human primates, Human

Immunogen

WERI-RB-1 retinoblastoma cells

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Leucocyte common antigen related molecule, PTPRF, W7C6

Antigen description

LAR is a receptore-linked transmembrane protein tyrosine phosphatase expressed on mesenchymal stem cells, that reside e.g. in bone marrow, blood, placenta, adipose tissue, or skin, as well as it is expressed on some carcinoma cell lines, including HeLa, MCF-7, or HT29. During the process of externalization, LAR is intracellularly proteolytically processed into two non-covalently associated subunits. This protein is involved in intercellular and cell-matrix interactions and its extracellular part resembles that of cell adhesion molecules (CAMs). The extracellular part can be released from the surface, which may be used for regulation of LAR function.

Entrez Gene ID 5792

UniProt ID G1UI20

Separation of HeLa cells stained using anti-human LAR (W7C6) APC antibody (concentration in sample 1,7 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1,7 μg/ml, same as LAR APC concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.

Product specific references:

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed