Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu HER3 Purified

Anti-Hu HER3 Purified

Regulatory status

RUO

Antigen

HER3

Clone

H3Mab-17

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-901-C100

In stock

154.00 USD

Variant

0.1 mg

11-901-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody H3Mab-17 recognizes an extracellular epitope on human HER3, a member of EGFR family of receptor tyrosine kinases, which is overexpressed in many cancers.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

HER3-transfected CHO cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ERBB3, FERLK, LCCS2

Antigen description

HER3 (ERBB3) is a transmembrane protein of the epidermal growth factor receptor family, although it does not have an active tyrosine kinase domain. It can bind its ligand, but for further signaling it needs heterodimerization with other receptor tyrosine kinases of EGFR family. Overexpression of HER3 has been observed in many carcinomas. Activity of HER3 can be modulated by one of its isoforms, that is secreted from the cell, as its lacks the transmembrane domain.

Entrez Gene ID 2065

UniProt ID P21860

Separation of MCF-7 cells stained using anti-HER3 (H3Mab-17) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).