Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu HER3 PE-Cy™7

Anti-Hu HER3 PE-Cy™7

Regulatory status

RUO

Antigen

HER3

Clone

H3Mab-17

Format

PE-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-901-T100

In stock

282.70 USD

Variant

100 tests

T7-901-T100

In stock

282.70 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody H3Mab-17 recognizes an extracellular epitope on human HER3 (erbB3), a member of EGFR family of receptor tyrosine kinases, which is overexpressed in many cancers.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

HER3-transfected CHO cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

ERBB3, FERLK, LCCS2

Antigen description

HER3 (ERBB3) is a transmembrane protein of the epidermal growth factor receptor family, although it does not have an active tyrosine kinase domain. It can bind its ligand, but for further signaling it needs heterodimerization with other receptor tyrosine kinases of EGFR family. Overexpression of HER3 has been observed in many carcinomas. Activity of HER3 can be modulated by one of its isoforms, that is secreted from the cell, as its lacks the transmembrane domain.

Entrez Gene ID 2065

UniProt ID P21860

Separation of MCF-7 cells stained using anti-human HER3 (H3Mab-17) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml, red-filled) from MCF-7 cells stained using mouse IgG2a isotype control (MOPC-173) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml, same as HER3 PE-Cy™7 concentration, black-dashed) in flow cytometry analysis (surface staining).