Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu Galectin-9 PE

Anti-Hu Galectin-9 PE

Regulatory status

RUO

Antigen

Galectin-9

Clone

9M1-3

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-928-T100

In stock

242.00 USD

Variant

100 tests

1P-928-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 9M1-3 recognizes an epitope within C terminus of human galectin-9, a glycan-binding protein expressed mainly on activated Th cells and FoxP3+ Treg cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Non-human primates, Human

Immunogen

recombinant M-type splicing variant of human galectin 9

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HUAT, LGalS9A, LEG9

Antigen description

Galectin-9 is a glycan-binding protein, which is expressed in three main isoforms of 49 aa, 27 aa, and 15 aa. It can be detected on the cell surface, as well as intracellularly, or in a secreted form. On the cell surface, galectin-9 plays roles in contacts with other cells and with extracellular matrix. It is expressed on multiple cell types, but mainly on Treg cells, activated Th cells and some cancers. Its secreted form acts like a cytokine with immunomodulatory and immunosuppresive functions. Massive and inadequate production of galectin-9, associated with some viral infections or cancers, can counteract immune reactions to these illnesses. High levels of galectin-9 expression lead to poor prognosis of cancer patients.

Entrez Gene ID 3965

UniProt ID O00182

Separation of Jurkat cells stained using anti-human Galectin-9 (9M1-3) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension, red-filled) from Jurkat cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as Galectin-9 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Chen Z, Dong D, Zhu Y, Pang N, Ding J: The role of Tim-3/Galectin-9 pathway in T-cell function and prognosis of patients with human papilloma virus-associated cervical carcinoma. FASEB J. 2021 Mar;35(3):e21401.
PubMed

Motamedi M, Shahbaz S, Fu L, Dunsmore G, Xu L, Harrington R, Houston S, Elahi S: Galectin-9 expression defines a subpopulation of NK cells with impaired cytotoxic effector molecules but enhanced IFN-γ production, dichotomous to TIGIT, in HIV-1 infection. Immunohorizons. 2019 Nov 15;3(11):531-546.
PubMed

Bertino P, Premeaux TA, Fujita T, Haun BK, Marciel MP, Hoffmann FW, Garcia A, Yiang H, Pastorino S, Carbone M, Niki T, Berestecky J, Hoffmann PR, Novu LC: Targeting the C-terminus of galectin-9 induces mesothelioma apoptosis and M2 macrophage depletion. Oncoimmunology 2019 Apr 17;8(8):1601482.
PubMed

Klibi J, Niki T, Riedel A, Pioche-Durieu C, Souquere S, Rubinstein E, Le Moulec S, Guigay J, Hirashima M, Guemira F, Adhikary D, Mautner J, Busson P: Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells. Blood 2009 Feb 26;113(9):1957-66.
PubMed

Lhuillier C, Barjon C, Baloche V, Niki T, Gelin A, Mustapha R, Claër L, Hoos S, Chiba Y, Ueno M, Hirashima M, Wei M, Morales O, Raynal B, Delhem N, Dellis O, Busson P: Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9. PLoS One 2018 Sep 11;13(9):e0202512.
PubMed