Anti-Hu Galectin-9 Alexa Fluor® 647

Anti-Hu Galectin-9 Alexa Fluor<sup>®</sup> 647
Regulatory status
RUO
Antigen
Galectin-9
Clone
9M1-3
Format
Alexa Fluor® 647
Reactivity
Non-human primates, Human
Excitation laser
red (633 nm)
Variant
100 tests
A6-928-T100
In stock
242.00 USD
Variant
100 tests
A6-928-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 9M1-3 recognizes an epitope within C terminus of human galectin-9, a glycan-binding protein expressed mainly on activated Th cells and FoxP3+ Treg cells.
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Human
Immunogen
recombinant M-type splicing variant of human galectin 9
Preparation
Purified antibody is conjugated with Alexa Fluor® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
HUAT, LGalS9A, LEG9
Antigen description
Galectin-9 is a glycan-binding protein, which is expressed in three main isoforms of 49 aa, 27 aa, and 15 aa. It can be detected on the cell surface, as well as intracellularly, or in a secreted form. On the cell surface, galectin-9 plays roles in contacts with other cells and with extracellular matrix. It is expressed on multiple cell types, but mainly on Treg cells, activated Th cells and some cancers. Its secreted form acts like a cytokine with immunomodulatory and immunosuppresive functions. Massive and inadequate production of galectin-9, associated with some viral infections or cancers, can counteract immune reactions to these illnesses. High levels of galectin-9 expression lead to poor prognosis of cancer patients.
Entrez Gene ID 3965
UniProt ID O00182
A6-928_FC_Histogram
Separation of Jurkat cells stained using anti-human Galectin-9 (9M1-3) Alexa Fluor® 647 antibody (4 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml, same as Galectin-9 Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Chen Z, Dong D, Zhu Y, Pang N, Ding J: The role of Tim-3/Galectin-9 pathway in T-cell function and prognosis of patients with human papilloma virus-associated cervical carcinoma. FASEB J. 2021 Mar;35(3):e21401.
PubMed
Motamedi M, Shahbaz S, Fu L, Dunsmore G, Xu L, Harrington R, Houston S, Elahi S: Galectin-9 expression defines a subpopulation of NK cells with impaired cytotoxic effector molecules but enhanced IFN-γ production, dichotomous to TIGIT, in HIV-1 infection. Immunohorizons. 2019 Nov 15;3(11):531-546.
PubMed
Bertino P, Premeaux TA, Fujita T, Haun BK, Marciel MP, Hoffmann FW, Garcia A, Yiang H, Pastorino S, Carbone M, Niki T, Berestecky J, Hoffmann PR, Novu LC: Targeting the C-terminus of galectin-9 induces mesothelioma apoptosis and M2 macrophage depletion. Oncoimmunology 2019 Apr 17;8(8):1601482.
PubMed
Klibi J, Niki T, Riedel A, Pioche-Durieu C, Souquere S, Rubinstein E, Le Moulec S, Guigay J, Hirashima M, Guemira F, Adhikary D, Mautner J, Busson P: Blood diffusion and Th1-suppressive effects of galectin-9-containing exosomes released by Epstein-Barr virus-infected nasopharyngeal carcinoma cells. Blood 2009 Feb 26;113(9):1957-66.
PubMed
Lhuillier C, Barjon C, Baloche V, Niki T, Gelin A, Mustapha R, Claër L, Hoos S, Chiba Y, Ueno M, Hirashima M, Wei M, Morales O, Raynal B, Delhem N, Dellis O, Busson P: Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9. PLoS One 2018 Sep 11;13(9):e0202512.
PubMed
Variant
100 tests
A6-928-T100
In stock
242.00 USD
Variant
100 tests
A6-928-T100
In stock
242.00 USD

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