Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu Fc epsilon RIa Purified

Anti-Hu Fc epsilon RIa Purified

Regulatory status

RUO

Antigen

Fc epsilon RIa

Clone

AER-37 [CRA1]

Format

Purified

Reactivity

Human, Non-human primates

Application

ICC (reported), IHC-F (reported), FC (QC tested)

Variant

0.1 mg

11-959-C100

In stock

158.40 USD

Variant

0.1 mg

11-959-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody AER-37 [CRA1] recognizes an extracellular epitope of the high-affinity IgE receptor I alpha subunit (Fc epsilon RIa).

Application

ICC (reported), IHC-F (reported), FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human, Non-human primates

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FcεR1α, FCER1A

Antigen description

FcεRIα serves as the IgE Fc-binding subunit of the high-affinity IgE receptor, expressed on the surface of mast cells and basophils. It associates with one beta and two gamma subunits that contain ITAM sequences in their intracellular domains, that function as docking sites in the process of signalosome forming after FcεRI triggering. Attached IgE gives to the receptor specificity for particular antigens which are target of the type I allergen sensitivity or hypersensitivity reaction, and prolongs stability of the receptor on the cell surface. Under physiological conditions this system protects the body against helminths and other parasites and venom toxins, but when dysregulated, it can lead to undesired allergic reactions and anaphylactic shock.

Entrez Gene ID 2205

UniProt ID P12319

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Fc epsilon RIa (AER-37 [CRA1]) purified antibody (concentration in sample 5 μg/ml) GAM APC.

Separation of human Fc epsilon RIa positive events (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Fc epsilon RIa (AER-37 [CRA1]) purified antibody (concentration in sample 5 μg/ml) GAM APC.

Product specific references:

Metz M, Staubach P, Bauer A, Brehler R, Gericke J, Kangas M, Ashton-Chess J, Jarvis P, Georgiou P, Canvin J, Hillenbrand R, Erpenbeck VJ, Maurer M: Clinical efficacy of omalizumab in chronic spontaneous urticaria is associated with a reduction of FcεRI-positive cells in the skin. Theranostics 2017 Mar 6;7(5):1266-1276.
PubMed

Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M: IgE- and FcepsilonRI-mediated migration of human basophils. Int Immunol. 2005 Sep;17(9):1249-55.
PubMed

Morshed M, Hlushchuk R, Simon D, Walls AF, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon HU, Yousefi S: NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol. 2014 Jun 1;192(11):5314-23.
PubMed