Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CX3CR1 FITC

Anti-Hu CX3CR1 FITC

Regulatory status

RUO

Antigen

CX3CR1

Clone

2A9-1

Format

FITC

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-956-T100

In stock

203.50 USD

Variant

100 tests

1F-956-T100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b kappa

Specificity

The rat monoclonal antibody 2A9-1 recognizes an extracellular epitope of CX3CR1, an important regulator of leukocyte trafficking and immune system ballance.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

human CX3CR1 transfectants

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, fractalkine receptor

Antigen description

CX3CR1 (fractalkine receptor) serves as a regulator of leukocyte trafficking. Its ligand CX3CL1 (fractalkine) can serve bots as chemoatractant (soluble) and as adhesive molecule (membrane bound). Binding to the cell surface expressed ligand leads to rapid induction of firm adhesion of CX3CR1-positive cells without requiring selectin-mediated rolling or activation of integrins. This interaction is important for trafficking of cytotoxic effector lymphocytes regardless of their lineage and mode of target cell recognition, through inflamed endothelium. Similarly, it mediates monocyte/macrophage recruitment to inflamed atherosclerotic plaques, as well as in their homing to non-inflamed tissues, where they can differentiate into dendritic cells. CX3CR1 also promotes cell survival, plays a key role in brain microglia, synapse maturation and regulation of inflammatory response in the central nervous system, it acts as a negative regulator of angiogenesis, and serves as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi.

Entrez Gene ID 1524

UniProt ID P49238

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 μg/ml).

Separation of human CXCR1 positive CD3 negative lymphocytes (red-filled) from CXCR1 negative CD3 positive lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CX3CR1 (2A9-1) FITC antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Nishimura M, Umehara H, Nakayama T, Yoneda O, Hieshima K, Kakizaki M, Dohmae N, Yoshie O, Imai T: Dual functions of fractalkine/CX3C ligand 1 in trafficking of perforin+/granzyme B+ cytotoxic effector lymphocytes that are defined by CX3CR1 expression. J Immunol. 2002 Jun 15;168(12):6173-80.
PubMed

Nanki T, Imai T, Nagasaka K, Urasaki Y, Nonomura Y, Taniguchi K, Hayashida K, Hasegawa J, Yoshie O, Miyasaka N: Migration of CX3CR1-positive T cells producing type 1 cytokines and cytotoxic molecules into the synovium of patients with rheumatoid arthritis. Arthritis Rheum. 2002 Nov;46(11):2878-83.
PubMed

Béziat V, Duffy D, Quoc SN, Le Garff-Tavernier M, Decocq J, Combadière B, Debré P, Vieillard V: CD56brightCD16+ NK cells: A functional intermediate stage of NK cell differentiation. J Immunol. 2011 Jun 15;186(12):6753-61.
PubMed