Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CLEC2 PE

Anti-Hu CLEC2 PE

Regulatory status

RUO

Antigen

CLEC2

Clone

AYP1

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-892-T100

Ask for delivery term HERE

242.00 USD

Variant

100 tests

1P-892-T100

Ask for delivery term HERE

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody AYP1 recognizes an epitope within the extracellular part of CLEC2, a transmembrane glycoprotein expressed on activated plateles and on platelet microparticles.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

A recombinant extracellular domain of human CLEC2 (amino acids 68-229)

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC1B, CLEC2B, PRO1384, QDED721

Antigen description

CLEC2 (C-type lectin-like receptor 2) functions as a platelet receptor for the lymphatic endothelial marker, PDPN, and mediates platelet activation. Besides platelets, it can be found on myeloid cells and NK cells. CLEC2 functions also as an attachment factor for HIV-1 and facilitates its capture by platelets. Platelet-aggregating snake venom protein rhodocytin also binds to CLEC2.

Entrez Gene ID 51266

UniProt ID Q9P126

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CLEC2 (AYP1) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Gitz E, Pollitt AY, Gitz-Francois JJ, Alshehri O, Mori J, Montague S, Nash GB, Douglas MR, Gardiner EE, Andrews RK, Buckley CD, Harrison P, Watson SP: CLEC-2 expression is maintained on activated platelets and on platelet microparticles. Blood 2014 Oct 2;124(14):2262-70
PubMed

Sung PS, Huang TF, Hsieh SL: Extracellular vesicles from CLEC2-activated platelets enhance dengue virus-induced lethality via CLEC5A/TLR2. Nat Commun. 2019 Jun 3;10(1):2402
PubMed