Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD99R Purified

Anti-Hu CD99R Purified

Regulatory status

RUO

Antigen

CD99R

Clone

MEM-131

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-236-C100

In stock

154.00 USD

0.025 mg

11-236-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-236-C100

In stock

154.00 USD

0.025 mg

11-236-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody MEM-131 reacts with CD99R, an extracellular epitope restricted to a subset of CD99 molecule expressed on myeloid cells, NK cells and T lymphocytes.

Workshop

HLDA V: WS Code AS S020; HLDA V: WS Code T T-E2.02; HLDA V: WS Code T T-017

Application

IP, WB, FC (QC tested), IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymic lymphocytes.
Flow cytometry: Recommended dilution: 1 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Human

Immunogen

HPB-ALL human peripheral blood leukemia T-cell line

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MIC2, HBA71, blood group Xg

Antigen description

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD99R (MEM-131) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99R (MEM-131) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

General references:

Bernard G, Raimondi V, Alberti I, Pourtein M, Widjenes J, Ticchioni M, Bernard A: CD99 (E2) up-regulates alpha4beta1-dependent T cell adhesion to inflamed vascular endothelium under flow conditions. Eur J Immunol. 2000 Oct;30(10):3061-5.
PubMed

Bernard G, Breittmayer JP, de Matteis M, Trampont P, Hofman P, Senik A, Bernard A: Apoptosis of immature thymocytes mediated by E2/CD99. J Immunol. 1997 Mar 15;158(6):2543-50.
PubMed

Bernard G, Zoccola D, Deckert M, Breittmayer JP, Aussel C, Bernard A: The E2 molecule (CD99) specifically triggers homotypic aggregation of CD4+ CD8+ thymocytes. J Immunol. 1995 Jan 1;154(1):26-32.
PubMed

Gelin C, Aubrit F, Phalipon A, Raynal B, Cole S, Kaczorek M, Bernard A: The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product. EMBO J. 1989 Nov;8(11):3253-9.
PubMed

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Olweus J, Lund-Johansen F, Terstappen LW: CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells. Blood. 1995 May 1;85(9):2402-13.
PubMed

Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed