Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD96 APC

Anti-Hu CD96 APC

Regulatory status

RUO

Antigen

CD96

Clone

NK92.39

Format

APC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-852-T100

In stock

242.00 USD

Variant

100 tests

1A-852-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody NK92.39 recognizes an extracellular epitope of CD96, a transmembrane glycoprotein expressed at low levels on resting, and at high levels on activated T and NK cells, as well as on many T cell leukemias.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

human NK92 cell line

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TACTILE, MGC22596

Antigen description

CD96 is a 160-240 kDa type I transmembrane glycoprotein with intracellular ITIM-like domain, however, without observed inhibitory function. This protein is involved in adhesive interactions of T and NK cells during the late phase of the immune response, and are expressed above all upon their activation. Strong interaction between CD96 and CD155 (poliovirus receptor) stimulates cytotoxicity of activated NK cells, which may play a role in NK cell recognition and killing of CD155-expressing tumor cells. CD96 is expressed on approximately 80% of T cell leukemias and 30% of acute myeloid leukemias and is being used for their immunophenotyping.

Entrez Gene ID 10225

UniProt ID Q8WUE2

Separation of PHA stimulated lymphocytes stained using anti-human CD96 (NK92.39) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from PHA stimulated lymphocytes stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as Galectin-3 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood cells.

Product specific references:

Eriksson EM, Keh CE, Deeks SG, Martin JN, Hecht FM, Nixon DF: Differential expression of CD96 surface molecule represents CD8⁺ T cells with dissimilar effector function during HIV-1 infection. PLoS One. 2012;7(12):e51696.
PubMed

Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M: Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J Immunol. 2004 Apr 1;172(7):3994-8.
PubMed