Anti-Hu CD95 Purified Azide Free

Anti-Hu CD95 Purified Azide Free
Regulatory status
RUO
Antigen
CD95
Clone
UT-1
Format
Purified Azide Free
Reactivity
Human
Application
FC (QC tested), FUNC
Variant
0.1 mg
10-395-C100
In stock

0.025 mg
10-395-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody.
Application
FC (QC tested), FUNC
Reactivity
Human
Immunogen
HUT-78 human T cell lymphoma cells
Other names
FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6
Preparation
Purified by precipitation and chromatography
Formulation
Azide free HEPES buffered saline (HBS) solution, 0.2 μm filter sterilized
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
10-395 FUNC
Functional application example: Induction of apoptosis in various hematopoetic cell lines by use of anti-Fas antibodies. Suspensions of used cell lines were incubated with soluble (or without as control) anti-Fas antibodies. Induction of apoptosis was measured by Apoptosis Assay Kit - FITC. Histograms: Red (full) – untreated control cells Black – standard apoptosis-inducing anti-Fas antibody Green – anti-Fas (UT-1) A – JURKAT human peripheral blood T cell leukemia cell line B – TF-1 human bone marrow erythroleukemia cell line C – CEM human leukemia cell line D – MOLT-4 human acute lymphoblastic T cell leukemia cell line

General references:

Brumatti G, Yon M, Castro FA, Bueno-da-Silva AE, Jacysyn JF, Brunner T, Amarante-Mendes GP: Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide. Exp Cell Res. 2008 Feb 1;314(3):554-63.
PubMed
Guo Z, Zhang M, Tang H, Cao X: Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines. Blood. 2005 Sep 15;106(6):2033-41.
PubMed
Park DR, Thomsen AR, Frevert CW, Pham U, Skerrett SJ, Kiener PA, Liles WC: Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages. J Immunol. 2003 Jun 15;170(12):6209-16.
PubMed
Scaffidi C, Fulda S, Srinivasan A, Friesen C, Li F, Tomaselli KJ, Debatin KM, Krammer PH, Peter ME: Two CD95 (APO-1/Fas) signaling pathways. EMBO J. 1998 Mar 16;17(6):1675-87.
PubMed

Product specific references:

Matsuyama W, Mitsuyama H, Ono M, Shirahama Y, Higashimoto I, Osame M, Arimura K: Discoidin domain receptor 1 contributes to eosinophil survival in an NF-kappaB-dependent manner in Churg-Strauss syndrome. Blood. 2007 Jan 1;109(1):22-30.
PubMed
Variant
0.1 mg
10-395-C100
In stock

0.025 mg
10-395-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock