Anti-Hu CD92 Purified

Anti-Hu CD92 Purified
Regulatory status
RUO
Antigen
CD92
Clone
VIM15
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-866-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody VIM15 recognizes an extracellular epitope of CD92, a 70 kDa transmembrane glycoprotein expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines.
Workshop
HLDA VI: M-MR6, HLDA V: BP-BP229, M-M15
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
MV4-11 acute myeloid leukemia cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CTL1, CHTL1, SLC44A1
Antigen description
CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.
Entrez Gene ID 23446
UniProt ID Q8WWI5
11-866_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD92 (VIM15) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
11-866_FC_Histogram
Separation of monocytes stained anti-human CD92 (VIM15) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Wille S, Szekeres A, Majdic O, Prager E, Staffler G, Stöckl J, Kunthalert D, Prieschl EE, Baumruker T, Burtscher H, Zlabinger GJ, Knapp W, Stockinger H: Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells. J Immunol. 2001 Nov 15;167(10):5795-804.
PubMed
Variant
0.1 mg
11-866-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD