Anti-Hu CD90 Purified Azide Free

Anti-Hu CD90 Purified Azide Free
Regulatory status
RUO
Antigen
CD90
Clone
5E10
Format
Purified Azide Free
Reactivity
Pig, Horse, Human, Non-human primates
Variant
0.1 mg
10-652-C100
In stock
143.00 USD

0.025 mg
10-652-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
10-652-C100
In stock
143.00 USD

0.025 mg
10-652-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.
Workshop
HLDA V: WS Code M07, BP222; HLDA VI: WS Code BP28, E046
Application details
Flow cytometry: Recommended dilution: 1-3 μg/ml.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Reactivity
Pig, Horse, Human, Non-human primates
Negative species
Dog
Immunogen
HEL erythroleukemia cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Thy-1
Antigen description
CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.
Entrez Gene ID 7070
UniProt ID P04216
10-652_FC_Histogram
Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD90 (5E10) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Product specific references:

Chin-Yee IH, Keeney M, Stewart AK, Belch A, Bence-Buckler I, Couban S, Howson-Jan K, Rubinger M, Stewart D, Sutherland R, Paragamian V, Bhatia M, Foley R: Optimising parameters for peripheral blood leukapheresis after r-metHuG-CSF (filgrastim) and r-metHuSCF (ancestim) in patients with multiple myeloma: a temporal analysis of CD34(+) absolute counts and subsets. Bone Marrow Transplant. 2002 Dec;30(12):851-60.
PubMed
Hoppstädter J, Diesel B, Zarbock R, Breinig T, Monz D, Koch M, Meyerhans A, Gortner L, Lehr CM, Huwer H, Kiemer AK: Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages. Respir Res. 2010 Sep 15;11:124.
PubMed
Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed
Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S: Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES. J Invest Dermatol. 2009 Jun;129(6):1569-81.
PubMed
Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. Blood. 2001 May 1;97(9):2741-9.
PubMed
Ito T, Inaba M, Inaba K, Toki J, Sogo S, Iguchi T, Adachi Y, Yamaguchi K, Amakawa R, Valladeau J, Saeland S, Fukuhara S, Ikehara S: A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells. J Immunol. 1999 Aug 1;163(3):1409-19.
PubMed
St Louis DC, Woodcock JB, Franzoso G, Blair PJ, Carlson LM, Murillo M, Wells MR, Williams AJ, Smoot DS, Kaushal S, Grimes JL, Harlan DM, Chute JP, June CH, Siebenlist U, Lee KP: Evidence for distinct intracellular signaling pathways in CD34+ progenitor to dendritic cell differentiation from a human cell line model. J Immunol. 1999 Mar 15;162(6):3237-48.
PubMed
Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 2012;21(10):2201-14.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Variant
0.1 mg
10-652-C100
In stock
143.00 USD

0.025 mg
10-652-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
10-652-C100
In stock
143.00 USD

0.025 mg
10-652-C025
Delivery 1 week
71.50 USD

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