Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD9 Purified

Anti-Hu CD9 Purified

Regulatory status

RUO

Antigen

CD9

Clone

MEM-61

Format

Purified

Reactivity

Human

Application

MC (CyTOF), WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-208-C100

In stock

130.00 USD

0.025 mg

11-208-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-208-C100

In stock

130.00 USD

0.025 mg

11-208-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Workshop

HLDA VI: WS Code P P-15

Application

MC (CyTOF), WB, FC (QC tested), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 2-4 μg/ml, non-reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/ml, positive tissue: prostate.

Reactivity

Human

Immunogen

Pre-B cell line NALM-6.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, BA2

Antigen description

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Entrez Gene ID 928

UniProt ID P21926

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD9 (MEM-61) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Separation of CD9 positive thrombocytes (red-filled) from human CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD9 (MEM-61) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Mass cytometry (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.

General references:

Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9.
PubMed

Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96.
PubMed

Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7.
PubMed

Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65.
PubMed

Product specific references:

Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33.
PubMed

Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov;293(5):C1660-8.
PubMed

Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr;20(7):2030-40.
PubMed

Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).