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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD89 PE
Anti-Hu CD89 PE
Regulatory status
RUO
Antigen
CD89
Clone
A59
Format
PE
Reactivity
Human, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-139-T100
In stock
248.60 USD
25 tests
1P-139-T025
Delivery 1 week
124.30 USD
Variant
100 tests
1P-139-T100
In stock
248.60 USD
25 tests
1P-139-T025
Delivery 1 week
124.30 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody A59 recognizes an extracellular epitope of CD89, a 55-100 kDa glycoprotein serving as a receptor for IgA and expressed mainly on granulocytes, monocytes and macrophages.
Workshop
HLDA: WS Code V MR30
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
Ag8.653 myeloma cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
FcalphaRI, FcAR, CTB-61M7.2
Antigen description
CD89 (Fc-alpha-R) is a type I transmembrane glycoprotein serving as a receptor for IgA. Soluble CD89 is detectable in serum and retains its IgA binding capacity. For signal transduction the association with FcR gamma chain homodimers is needed. CD89 is expressed on granulocytes, monocytes, macrophages, dendritic cells and myeloid cell lines. Its expression is upregulated in presence of IgA immune complexes, stimulators (such as LPS, PMA), TNF alpha, IL1 beta or GM-CSF, and it is downregulated in presence of TGF beta and suramin. Binding of IgA-opsonized targets to CD89 leads to phagocytic and cytotoxic processes of the immunologic defense.
Entrez Gene ID
2204
UniProt ID
P24071
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD89 (A59) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD89 (A59) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
Product specific references:
Barratt J, Greer MR, Pawluczyk IZ, Allen AC, Bailey EM, Buck KS, Feehally J: Identification of a novel Fcalpha receptor expressed by human mesangial cells. Kidney Int. 2000 May;57(5):1936-48.
PubMed
Wu J, Ji C, Xie F, Langefeld CD, Qian K, Gibson AW, Edberg JC, Kimberly RP: FcalphaRI (CD89) alleles determine the proinflammatory potential of serum IgA. J Immunol. 2007 Mar 15;178(6):3973-82.
PubMed
Rogers KA, Scinicariello F, Attanasio R: Identification and characterization of macaque CD89 (immunoglobulin A Fc receptor). Immunology. 2004 Oct;113(2):178-86.
PubMed
van Dijk TB, Bracke M, Caldenhoven E, Raaijmakers JA, Lammers JW, Koenderman L, de Groot RP: Cloning and characterization of Fc alpha Rb, a novel Fc alpha receptor (CD89) isoform expressed in eosinophils and neutrophils. Blood. 1996 Dec 1;88(11):4229-38.
PubMed
van Egmond M, van Spriel AB, Vermeulen H, Huls G, van Garderen E, van de Winkel JG: Enhancement of polymorphonuclear cell-mediated tumor cell killing on simultaneous engagement of fcgammaRI (CD64) and fcalphaRI (CD89). Cancer Res. 2001 May 15;61(10):4055-60.
PubMed
Honorio-França AC, Launay P, Carneiro-Sampaio MM, Monteiro RC: Colostral neutrophils express Fc alpha receptors (CD89) lacking gamma chain association and mediate noninflammatory properties of secretory IgA. J Leukoc Biol. 2001 Feb;69(2):289-96.
PubMed
Schenk M, Bouchon A, Seibold F, Mueller C: TREM-1--expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J Clin Invest. 2007 Oct;117(10):3097-106.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
1P-139-T100
In stock
248.60 USD
25 tests
1P-139-T025
Delivery 1 week
124.30 USD
Variant
100 tests
1P-139-T100
In stock
248.60 USD
25 tests
1P-139-T025
Delivery 1 week
124.30 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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