Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD88 APC

Anti-Hu CD88 APC

Regulatory status

RUO

Antigen

CD88

Clone

S5/1

Format

APC

Reactivity

Rabbit, Ferret, Cow, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

1A-736-T100

In stock

242.00 USD

Variant

100 tests

1A-736-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody S5/1 recognizes an extracellular epitope of CD88 protein, a 43 kDa receptor of C5a component of the complement cascade.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Rabbit, Ferret, Cow, Human

Immunogen

Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

C5A, C5R1, C5AR1

Antigen description

CD88 / C5aR is a G protein-coupled seven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis.

Entrez Gene ID 728

UniProt ID P21730

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD88 (S5/1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD88 (S5/1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Kiener HP, Baghestanian M, Dominkus M, Walchshofer S, Ghannadan M, Willheim M, Sillaber C, Graninger WB, Smolen JS, Valent P: Expression of the C5a receptor (CD88) on synovial mast cells in patients with rheumatoid arthritis. Arthritis Rheum. 1998 Feb;41(2):233-45.
PubMed

Conway Morris A, Kefala K, Wilkinson TS, Dhaliwal K, Farrell L, Walsh T, Mackenzie SJ, Reid H, Davidson DJ, Haslett C, Rossi AG, Sallenave JM, Simpson AJ: C5a mediates peripheral blood neutrophil dysfunction in critically ill patients. Am J Respir Crit Care Med. 2009 Jul 1;180(1):19-28.
PubMed

Cain SA, Monk PN: The orphan receptor C5L2 has high affinity binding sites for complement fragments C5a and C5a des-Arg(74). J Biol Chem. 2002 Mar 1;277(9):7165-9.
PubMed

Camous L, Roumenina L, Bigot S, Brachemi S, Frémeaux-Bacchi V, Lesavre P, Halbwachs-Mecarelli L: Complement alternative pathway acts as a positive feedback amplification of neutrophil activation. Blood. 2011 Jan 27;117(4):1340-9.
PubMed