Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD87 Purified

Anti-Hu CD87 Purified

Regulatory status

RUO

Antigen

CD87

Clone

VIM5

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-792-C100

In stock

147.40 USD

0.025 mg

11-792-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

11-792-C100

In stock

147.40 USD

0.025 mg

11-792-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody VIM5 recognizes CD87 (urokinase plasminogen activator receptor), a 36-68 kDa single-chain GPI-anchored extracellular glycoprotein expressed on granulocytes, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts and keratinocytes.

Workshop

HLDA VI: WS Code MR13

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

human myeloid cell line THP-1

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

UPAR, URKR, PLAUR

Antigen description

CD87, the urokinase plasminogen activator receptor (UPAR), is a GPI-anchored single chain glycoprotein of a 50-68 kDa, which is expressed on granulocytes, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts and keratinocytes. The urokinase plasminogen activator bound to CD87 converts plasminogen to plasmin, and being concentrated on the leading edge of migrating cells, it plays important role in cell adhesion and chemotaxis. CD87 binds to β1, β2, and β3 integrins, and can contribute to cancer cell invasion and metastasis. This antigen can also be used to study normal and abnormal granulopoiesis.

Entrez Gene ID 5329

UniProt ID Q03405

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD87 (VIM5) purified antibody (concentration in sample 2 μg/ml) GAM APC.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD87 (VIM5) purified antibody (concentration in sample 2 μg/ml) GAM APC.

Product specific references:

Gleixner KV, Mayerhofer M, Sonneck K, Gruze A, Samorapoompichit P, Baumgartner C, Lee FY, Aichberger KJ, Manley PW, Fabbro D, Pickl WF, Sillaber C, Valent P: Synergistic growth-inhibitory effects of two tyrosine kinase inhibitors, dasatinib and PKC412, on neoplastic mast cells expressing the D816V-mutated oncogenic variant of KIT. Haematologica. 2007 Nov;92(11):1451-9.
PubMed

Lanza F, Castoldi GL, Castagnari B, Todd RF 3rd, Moretti S, Spisani S, Latorraca A, Focarile E, Roberti MG, Traniello S: Expression and functional role of urokinase-type plasminogen activator receptor in normal and acute leukaemic cells. Br J Haematol. 1998 Oct;103(1):110-23.
PubMed

Elghetany MT, Patel J, Martinez J, Schwab H: CD87 as a marker for terminal granulocytic maturation: assessment of its expression during granulopoiesis. Cytometry B Clin Cytom. 2003 Jan;51(1):9-13.
PubMed