Anti-Hu CD85k Purified

Anti-Hu CD85k Purified
Regulatory status
RUO
Antigen
CD85k
Clone
ZM4.1
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-906-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody ZM4.1 recognizes an extracellular epitope of human CD85k, a transmembrane glycoprotein expressed by monocytes, macrophages, and dendritic cells.
Application
Application details
Flow cytometry: Recommended dilution: 3-6 μg/ml.
Reactivity
Human
Immunogen
human CD85k
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
HM18, ILT3, LIR5, LILRB4
Antigen description
CD85k is a type I transmembrane glycoprotein of ILT/LIR family, and contains three ITIM motives, which recruit protein tyrosine phosphatases SHP-1 and SHP-2, resulting in supression of signal transduction. It is expressed by monocytes, macrophages, and dendritic cells. Antigen presenting cells expressing CD85k and CD85d appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.
Entrez Gene ID 11006
UniProt ID Q8NHJ6
11-906_FC_Dot-plot
Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using anti-human CD85k (ZM4.1) purified antibody (concentration in sample 5 μg/ml, GAM APC) and anti-human CD123 (6H6) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
11-906_FC_Histogram
Separation of human peripheral blood CD85k positive mononuclear cells (red-filled) from CD85k negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD85k (ZM4.1) purified antibody (concentration in sample 5 μg/ml, GAM APC.

Product specific references:

Waschbisch A, Sanderson N, Krumbholz M, Vlad G, Theil D, Schwab S, Mäurer M, Derfuss T: Interferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients. PLoS One 2014 Dec 31;9(12):e115488.
PubMed
Michalski J, Deinzer A, Stich L, Zinser E, Steinkasserer A, Knippertz I: Quercetin induces an immunoregulatory phenotype in maturing human dendritic cells. Immunobiology 2020 Jul;225(4):151929.
PubMed
Littringer K, Moresi C, Rakebrandt N, Zhou X, Schorer M, Dolowschiak T, Kirchner F, Rost F, Keller CW, McHugh D, Leibund G, Landmann S, Robinson MD, Joller N: Common features of regulatory T cell specialization during Th1 responses. Front Immunol. 2018 Jun 13;9:1344.
PubMed
Inui M, Sugahara-Tobinai A, Fujii H, Itoh-Nakadai A, Fukuyama H, Kurosaki T, Ishii T, Harigae H, Takai T: Tolerogenic immunoreceptor ILT3/LILRB4 paradoxically marks pathogenic auto-antibody-producing plasmablasts and plasma cells in non-treated SLE . Int Immunol. 2016 Dec;28(12):597-604.
PubMed
Chen HM, van der Touw W, Wang YS, Kang K, Mai S, Zhang J, Alsina-Beauchamp D, Duty JA, Mungamuri SK, Zhang B, Moran T, Flavell R, Aaronson S, Hu HM, Arase H, Ramanathan S, Flores R, Pan PY, Chen SH: Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity. J Clin Invest. 2018 Dec 3;128(12):5647-5662.
PubMed
Variant
0.1 mg
11-906-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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