Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD85j Purified Low Endotoxin

Anti-Hu CD85j Purified Low Endotoxin

Anti-Hu CD85j Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD85j
Clone
GHI/75
Format
Purified Low Endotoxin
Reactivity
Human
Application
IP, WB, FC (QC tested), FUNC
Variant
0.1 mg
12-740-C100
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
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Product details
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References
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
Application
IP, WB, FC (QC tested), FUNC
Reactivity
Human
Immunogen
Hairy cell leukaemia cells
Other names
ILT2, LIR1, MIR7, PIRB, ILT-2, LIR-1, MIR-7, PIR-B
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
12-740_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD85j(GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.
12-740_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD85j (GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
12-740_FC_Histogram
Separation of human CD85j positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85j(GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Product specific references:

Pulford K, Micklem K, Thomas J, Jones M, Mason DY: A 72-kD B cell-associated surface glycoprotein expressed at high levels in hairy cell leukaemia and plasma cell neoplasms. Clin Exp Immunol. 1991 Sep;85(3):429-35.
PubMed
Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb;13(2):193-201.
PubMed
Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed
Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun;65(6):841-5.
PubMed
Variant
0.1 mg
12-740-C100
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Contact us
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