Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD85j Purified Low Endotoxin

Anti-Hu CD85j Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD85j

Clone

GHI/75

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, WB, FC (QC tested), FUNC

Variant

0.1 mg

12-740-C100

In stock

130.00 USD

Variant

0.1 mg

12-740-C100

In stock

130.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).

Application

IP, WB, FC (QC tested), FUNC

Application details

Functional application: Blocking of HLA-G induced TGF-beta1 production.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

Hairy cell leukaemia cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ILT2, LIR1, MIR7, PIRB, ILT-2

Antigen description

CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.

Entrez Gene ID 10859

UniProt ID Q8NHL6

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD85j(GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD85j (GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD85j positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85j(GHI/75) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Product specific references:

Pulford K, Micklem K, Thomas J, Jones M, Mason DY: A 72-kD B cell-associated surface glycoprotein expressed at high levels in hairy cell leukaemia and plasma cell neoplasms. Clin Exp Immunol. 1991 Sep;85(3):429-35.
PubMed

Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb;13(2):193-201.
PubMed

Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed

Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun;65(6):841-5.
PubMed