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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD85j Purified
Anti-Hu CD85j Purified
Regulatory status
RUO
Antigen
CD85j
Clone
GHI/75
Format
Purified
Reactivity
Human
Application
IP, WB, FC (QC tested)
Variant
0.1 mg
11-740-C100
In stock
143.00 USD
0.025 mg
11-740-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-740-C100
In stock
143.00 USD
0.025 mg
11-740-C025
Delivery 1 week
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody GHI/75 recognizes an extracellular epitope of CD85j / ILT2, an 110-120 kDa membrane glycoprotein expressed strongly on plasma cells, moderately on circulating B cells, and weakly on monocytes. It is also expressed on T cell and NK cell subsets (variable, individual).
Application
IP, WB, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Hairy cell leukaemia cells
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ILT2, LIR1, MIR7, PIRB, ILT-2
Antigen description
CD85j, also known as ILT-2 (Ig-like transcript 2), LIR-1 (leukocyte Ig-like receptor 1), or LILRB1 (leukocyte Ig-like receptor B1), is a member of Ig superfamily transmembrane glycoproteins named CD85. The CD85j protein is expressed on several types of immune cells (plasma cells, B cells, monocytes, T and NK cell subsets) where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity.
Entrez Gene ID
10859
UniProt ID
Q8NHL6
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD85j (GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC and anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD85j positive B cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85j(GHI/75) purified antibody (concentration in sample 1 μg/ml) GAM APC.
Product specific references:
Pulford K, Micklem K, Thomas J, Jones M, Mason DY: A 72-kD B cell-associated surface glycoprotein expressed at high levels in hairy cell leukaemia and plasma cell neoplasms. Clin Exp Immunol. 1991 Sep;85(3):429-35.
PubMed
Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA-G1 co-expression boosts the HLA class I-mediated NK lysis inhibition. Int Immunol. 2001 Feb;13(2):193-201.
PubMed
Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13(9):822-30.
PubMed
Banham AH, Colonna M, Cella M, Micklem KJ, Pulford K, Willis AC, Mason DY: Identification of the CD85 antigen as ILT2, an inhibitory MHC class I receptor of the immunoglobulin superfamily. J Leukoc Biol. 1999 Jun;65(6):841-5.
PubMed
McIntire RH, Morales PJ, Petroff MG, Colonna M, Hunt JS: Recombinant HLA-G5 and -G6 drive U937 myelomonocytic cell production of TGF-beta1. J Leukoc Biol. 2004 Dec;76(6):1220-8.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-740-C100
In stock
143.00 USD
0.025 mg
11-740-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-740-C100
In stock
143.00 USD
0.025 mg
11-740-C025
Delivery 1 week
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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