Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD85h PE

Anti-Hu CD85h PE

Regulatory status

RUO

Antigen

CD85h

Clone

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-925-T100

In stock

242.00 USD

Variant

100 tests

1P-925-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 24 recognizes an extracellular epitope of CD85h, an approximately 70 kDa transmembrane glycoprotein.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

human CD85h transfectants

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ILT1, LIR7, LILRA2

Antigen description

CD85h (leukocyte Ig-like receptor A2) is a type I transmembrane glycoprotein, associating with Fc receptor gamma chain, to serve as an activating immunoreceptor. In the result, however, CD85h signaling leads to suppression of dendritic cell differentiation and antigen presentation, and of innate immune responses. This glycoprotein is expressed mainly on monocytes, macrophages, and myeloid dendritic cells, at a lower level on granulocyte and NK cell subsets.

Entrez Gene ID 11027

UniProt ID Q8N149

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85h (24) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85h (24) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36.
PubMed