Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD85g FITC

Anti-Hu CD85g FITC

Regulatory status

RUO

Antigen

CD85g

Clone

17G10.2

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-164-T100

In stock

198.00 USD

Variant

100 tests

1F-164-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 17G10.2 recognizes an extracellular epitope of CD85g / ILT7, a member of leukocyte immunoglobulin-like receptor family expressed on plasmacytoid dendritic cells, but not on myeloid dendritic cells and other peripheral blood leukocytes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ILT7, LILRA4

Antigen description

CD85g / ILT7 (immunoglobulin-like transcript 7) is a cell surface protein that is expressed on plasmacytoid dendritic cells (PDCs) and modulates the function of these cells in the immune response, such as the TLR-induced interferon production. It associates with gamma subunit of the high-affinity IgE receptor to form a receptor complex which transduces the signal through ITAM-associated downstream molecules. Expression of CD85g is downregulated by interleukin 3.

Entrez Gene ID 23547

UniProt ID P59901

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85g (17G10.2) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD123 positive CD85g positive leukocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85g (17G10.2) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Cao W, Rosen DB, Ito T, Bover L, Bao M, Watanabe G, Yao Z, Zhang L, Lanier LL, Liu YJ: Plasmacytoid dendritic cell-specific receptor ILT7-Fc epsilonRI gamma inhibits Toll-like receptor-induced interferon production. J Exp Med. 2006 Jun 12;203(6):1399-405.
PubMed

Tavano B, Galao RP, Graham DR, Neil SJ, Aquino VN, Fuchs D, Boasso A: Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes. J Immunol. 2013 Mar 15;190(6):2622-30.
PubMed

Tavano B, Boasso A: Effect of immunoglobin-like transcript 7 cross-linking on plasmacytoid dendritic cells differentiation into antigen-presenting cells. PLoS One. 2014 Feb 19;9(2):e89414.
PubMed