Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD85d PE-Cy™7

Anti-Hu CD85d PE-Cy™7

Regulatory status

RUO

Antigen

CD85d

Clone

42D1

Format

PE-Cy™7

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-907-T100

In stock

275.00 USD

Variant

100 tests

T7-907-T100

In stock

275.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 42D1 recognizes an extracellular epitope of human CD85d, a transmembrane glycoprotein expressed on NK cells, monocytes, macrophages, and weakly on granulocytes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

ILT4, LIR-2, LIR2, MIR-10, MIR10, LILRB2

Antigen description

CD85d is a type I transmembrane glycoprotein of the ILT/LIR family, which contains three intracellular ITIMs. It is expressed on NK cells, monocytes, macrophages, and weakly granulocytes. CD85d binds classic HLA class I molecules, and non-classic HLA-G1, and is involved in the suppression of NK cell-mediated cytotoxicity. Interaction between CD85d and trophoblast-specific HLA-G1 probably contributes to maternal tolerance of the fetus by inhibiting maternal leukocytes. Antigen presenting cells expressing CD85d and CD85k appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.

Entrez Gene ID 10288

UniProt ID Q8N423

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85d (42D1) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85d (42D1) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN: Characterization of human blood dendritic cell subsets. Blood 2002 Dec 15;100(13):4512-20.
PubMed

Lu N, Li Y, Zhang Z, Xing J, Sun Y, Yao S, Chen L: Human semaphorin-4A drives Th2 responses by binding to receptor ILT-4. Nat Commun. 2018 Feb 21;9(1):742.
PubMed

Sullivan RT, Kim CC, Fontana MF, Feeney ME, Jagannathan P, Boyle MJ, Drakeley CJ, Ssewanyana I, Nankya F, Mayanja-Kizza H, Dorsey G, Greenhouse B: FCRL5 delineates functionally impaired memory B cells associated with plasmodium falciparum exposure. PLoS Pathog. 2015 May 19;11(5):e1004894.
PubMed