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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD85d PE
Anti-Hu CD85d PE
Regulatory status
RUO
Antigen
CD85d
Clone
42D1
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-907-T100
In stock
242.00 USD
Variant
100 tests
1P-907-T100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody 42D1 recognizes an extracellular epitope of human CD85d, a transmembrane glycoprotein expressed on NK cells, monocytes, macrophages, and weakly on granulocytes.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ILT4, LIR-2, LIR2, MIR-10, MIR10, LILRB2
Antigen description
CD85d is a type I transmembrane glycoprotein of the ILT/LIR family, which contains three intracellular ITIMs. It is expressed on NK cells, monocytes, macrophages, and weakly granulocytes. CD85d binds classic HLA class I molecules, and non-classic HLA-G1, and is involved in the suppression of NK cell-mediated cytotoxicity. Interaction between CD85d and trophoblast-specific HLA-G1 probably contributes to maternal tolerance of the fetus by inhibiting maternal leukocytes. Antigen presenting cells expressing CD85d and CD85k appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.
Entrez Gene ID
10288
UniProt ID
Q8N423
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85d (42D1) PE antibody (4 μl reagent / 100 μl of peripheral whole blood).
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85d (42D1) PE antibody (4 μl reagent / 100 μl of peripheral whole blood).
Product specific references:
MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN: Characterization of human blood dendritic cell subsets. Blood 2002 Dec 15;100(13):4512-20.
PubMed
Lu N, Li Y, Zhang Z, Xing J, Sun Y, Yao S, Chen L: Human semaphorin-4A drives Th2 responses by binding to receptor ILT-4. Nat Commun. 2018 Feb 21;9(1):742.
PubMed
Sullivan RT, Kim CC, Fontana MF, Feeney ME, Jagannathan P, Boyle MJ, Drakeley CJ, Ssewanyana I, Nankya F, Mayanja-Kizza H, Dorsey G, Greenhouse B: FCRL5 delineates functionally impaired memory B cells associated with plasmodium falciparum exposure. PLoS Pathog. 2015 May 19;11(5):e1004894.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
1P-907-T100
In stock
242.00 USD
Variant
100 tests
1P-907-T100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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