Anti-Hu CD85d APC

Anti-Hu CD85d APC
Regulatory status
RUO
Antigen
CD85d
Clone
42D1
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-907-T100
In stock
242.00 USD
Variant
100 tests
1A-907-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody 42D1 recognizes an extracellular epitope of human CD85d, a transmembrane glycoprotein expressed on NK cells, monocytes, macrophages, and weakly on granulocytes.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ILT4, LIR-2, LIR2, MIR-10, MIR10, LILRB2
Antigen description
CD85d is a type I transmembrane glycoprotein of the ILT/LIR family, which contains three intracellular ITIMs. It is expressed on NK cells, monocytes, macrophages, and weakly granulocytes. CD85d binds classic HLA class I molecules, and non-classic HLA-G1, and is involved in the suppression of NK cell-mediated cytotoxicity. Interaction between CD85d and trophoblast-specific HLA-G1 probably contributes to maternal tolerance of the fetus by inhibiting maternal leukocytes. Antigen presenting cells expressing CD85d and CD85k appear to be crucial to the generation of CD8+ suppressor T cells and CD4+ regulatory T cells.
Entrez Gene ID 10288
UniProt ID Q8N423
1A-907_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD85d (42D1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1A-907_FC_Histogram
Separation of human monocytes (red-filled) from CD85d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD85d (42D1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

MacDonald KP, Munster DJ, Clark GJ, Dzionek A, Schmitz J, Hart DN: Characterization of human blood dendritic cell subsets. Blood 2002 Dec 15;100(13):4512-20.
PubMed
Lu N, Li Y, Zhang Z, Xing J, Sun Y, Yao S, Chen L: Human semaphorin-4A drives Th2 responses by binding to receptor ILT-4. Nat Commun. 2018 Feb 21;9(1):742.
PubMed
Sullivan RT, Kim CC, Fontana MF, Feeney ME, Jagannathan P, Boyle MJ, Drakeley CJ, Ssewanyana I, Nankya F, Mayanja-Kizza H, Dorsey G, Greenhouse B: FCRL5 delineates functionally impaired memory B cells associated with plasmodium falciparum exposure. PLoS Pathog. 2015 May 19;11(5):e1004894.
PubMed
Variant
100 tests
1A-907-T100
In stock
242.00 USD
Variant
100 tests
1A-907-T100
In stock
242.00 USD

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