Anti-Hu CD84 FITC

Anti-Hu CD84 FITC
Regulatory status
RUO
Antigen
CD84
Clone
CD84.1.21
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-661-T100
In stock
198.00 USD

25 tests
1F-661-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-661-T100
In stock
198.00 USD

25 tests
1F-661-T025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody CD84.1.21 recognizes an extracellular epitope of CD84, a single chain cell surface glycoprotein of 64-82 kDa, predominantly expressed B cells, monocytes, platelets and some T cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
CD84-transfected 300.19 cell line
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
LY9B, SLAMF5
Antigen description
CD84 is a highly glycosylated homophilic receptor of SLAM family. It is expressed on platelets and various types of leukocytes, especially following their activation. Ligation of CD84 leads to its phosphorylation on tyrosine residues within the cytoplasmic tail. These docking sites are recognized by downstream signaling molecules, such as phosphatase SHP-2 and adaptor protein SAP/SH2D1A. The function of CD84 has not been fully elucidated yet. Although predominantly activating receptor, its modulating activity was also demonstrated.
Entrez Gene ID 8832
UniProt ID Q9UIB8
1F-661_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD84 (CD84.1.21) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1F-661_FC_Histogram
Separation of human CD177 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD84 (CD84.1.21) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P: CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse. J Immunol. 2005 Jun 1;174(11):7033-42.
PubMed
Morra M, Simarro-Grande M, Martin M, Chen AS, Lanyi A, Silander O, Calpe S, Davis J, Pawson T, Eck MJ, Sumegi J, Engel P, Li SC, Terhorst C: Characterization of SH2D1A missense mutations identified in X-linked lymphoproliferative disease patients. J Biol Chem. 2001 Sep 28;276(39):36809-16.
PubMed
Morra M, Lu J, Poy F, Martin M, Sayos J, Calpe S, Gullo C, Howie D, Rietdijk S, Thompson A, Coyle AJ, Denny C, Yaffe MB, Engel P, Eck MJ, Terhorst C: Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. EMBO J. 2001 Nov 1;20(21):5840-52.
PubMed
Martin M, Romero X, de la Fuente MA, Tovar V, Zapater N, Esplugues E, Pizcueta P, Bosch J, Engel P: CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1. J Immunol. 2001 Oct 1;167(7):3668-76.
PubMed
Sayós J, Martín M, Chen A, Simarro M, Howie D, Morra M, Engel P, Terhorst C: Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP. Blood. 2001 Jun 15;97(12):3867-74.
PubMed
Variant
100 tests
1F-661-T100
In stock
198.00 USD

25 tests
1F-661-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-661-T100
In stock
198.00 USD

25 tests
1F-661-T025
Delivery 1 week
99.00 USD

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