Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD83 PE

Anti-Hu CD83 PE

Regulatory status

RUO

Antigen

CD83

Clone

HB15e

Format

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-677-T100

In stock

242.00 USD

25 tests

1P-677-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-677-T100

In stock

242.00 USD

25 tests

1P-677-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody HB15e recognizes an extracellular epitope of CD83, a 40-45 kDa type I glycoprotein expressed on mature dendritic cells.

Workshop

HLDA IV: WS Code T 85

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

Human CD83-transfected Cos cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

BL11, HB15

Antigen description

CD83 is a 40-45 kDa heavily glycosylated type I cell surface glycoprotein of immunoglobulin family. It is expressed on the surface of mature dendritic cells, Langerhans cells in the skin, and interdigitating reticulum cells in the lymphoid tissues. Low expression of CD83 has been reported in activated T and B cells. Cytoplasmic expression of CD83 can be detected also in monocytes and macrophages. CD83 is involved in modulation of antigen presentation. Soluble CD83 has immunoregulatory functions, it is able to down-regulate dendritic cell maturation and stimulation of T cells. In the developing immune system, release of soluble CD83 from dendritic cells upon stimulation by gram-positive or gram-negative bacteria has anti-allergic effect. Herpes simplex virus, on the other hand, causes CD83 degradation in mature dendritic cells.

Entrez Gene ID 9308

UniProt ID Q01151

Flow cytometry analysis (surface staining) of CD83 in IFN alpha-activated human peripheral blood cells with anti-CD83 (HB15e) PE.

Product specific references:

Loré K, Sönnerborg A, Broström C, Goh LE, Perrin L, McDade H, Stellbrink HJ, Gazzard B, Weber R, Napolitano LA, van Kooyk Y, Andersson J: Accumulation of DC-SIGN+CD40+ dendritic cells with reduced CD80 and CD86 expression in lymphoid tissue during acute HIV-1 infection. AIDS. 2002 Mar 29;16(5):683-92.
PubMed

Kummer M, Turza NM, Muhl-Zurbes P, Lechmann M, Boutell C, Coffin RS, Everett RD, Steinkasserer A, Prechtel AT: Herpes simplex virus type 1 induces CD83 degradation in mature dendritic cells with immediate-early kinetics via the cellular proteasome. J Virol. 2007 Jun;81(12):6326-38.
PubMed

Lundell AC, Andersson K, Josefsson E, Steinkasserer A, Rudin A: Soluble CD14 and CD83 from human neonatal antigen-presenting cells are inducible by commensal bacteria and suppress allergen-induced human neonatal Th2 differentiation. Infect Immun. 2007 Aug;75(8):4097-104.
PubMed

Klein E, Koch S, Borm B, Neumann J, Herzog V, Koch N, Bieber T: CD83 localization in a recycling compartment of immature human monocyte-derived dendritic cells. Int Immunol. 2005 Apr;17(4):477-87.
PubMed

Cao W, Lee SH, Lu J: CD83 is preformed inside monocytes, macrophages and dendritic cells, but it is only stably expressed on activated dendritic cells. Biochem J. 2005 Jan 1;385(Pt 1):85-93.
PubMed

Gerlini G, Mariotti G, Chiarugi A, Di Gennaro P, Caporale R, Parenti A, Cavone L, Tun-Kyi A, Prignano F, Saccardi R, Borgognoni L, Pimpinelli N: Induction of CD83+CD14+ nondendritic antigen-presenting cells by exposure of monocytes to IFN-alpha. J Immunol. 2008 Sep 1;181(5):2999-3008.
PubMed

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Holmstrøm K, Pedersen AW, Claesson MH, Zocca MB, Jensen SS: Identification of a microRNA signature in dendritic cell vaccines for cancer immunotherapy. Hum Immunol. 2010 Jan;71(1):67-73.
PubMed

Wang Y, Dennehy PH, Keyserling HL, Tang K, Gentsch JR, Glass RI, Jiang B: Rotavirus infection alters peripheral T-cell homeostasis in children with acute diarrhea. J Virol. 2007 Apr;81(8):3904-12.
PubMed