Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD82 Purified Low Endotoxin

Anti-Hu CD82 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD82

Clone

C33

Format

Purified Low Endotoxin

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(P), ICC, FUNC

Variant

0.1 mg

12-559-C100

In stock

150.00 USD

Variant

0.1 mg

11-261-C100

In stock

150.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Application

IP, WB, FC (QC tested), IHC(P), ICC, FUNC

Application details

Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

C91/PL (human HTLV-1+ T cell line)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography

Formulation

Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

Antigen description

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Entrez Gene ID 3732

UniProt ID P27701

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD82 (C33) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Separation of human CD82 positive lymphocytes (red-filled) from CD82 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD82 (C33) purified antibody (low endotoxin, concentration in sample 1 μg/ml) GAM APC.

Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33). Commercially tested by LifeSpan BioSciences.

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed

Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149(5):1435-40.
PubMed

Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7.
PubMed

Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39.
PubMed

Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81.
PubMed

Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401.
PubMed

Variant

0.1 mg

12-559-C100

In stock

150.00 USD

Variant

0.1 mg

11-261-C100

In stock

150.00 USD

Contact distributor

Datasheet download