Anti-Hu CD82 FITC

Anti-Hu CD82 FITC
Regulatory status
RUO
Antigen
CD82
Clone
C33
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-559-T100
In stock
198.00 USD

25 tests
1F-559-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-559-T100
In stock
198.00 USD

25 tests
1F-559-T025
Delivery 1 week
99.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
C91/PL (human HTLV-1+ T cell line)
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27
Antigen description
CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Entrez Gene ID 3732
UniProt ID P27701
1F-559 FC
Flow cytometry analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149(5):1435-40.
PubMed
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7.
PubMed
Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39.
PubMed
Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81.
PubMed
Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401.
PubMed
Variant
100 tests
1F-559-T100
In stock
198.00 USD

25 tests
1F-559-T025
Delivery 1 week
99.00 USD
Variant
100 tests
1F-559-T100
In stock
198.00 USD

25 tests
1F-559-T025
Delivery 1 week
99.00 USD

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