Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD81 Purified Low Endotoxin

Anti-Hu CD81 Purified Low Endotoxin

Anti-Hu CD81 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD81
Clone
M38
Format
Purified Low Endotoxin
Reactivity
Rabbit, Cat, Human
Application
IP, WB, FC (QC tested), IHC(P), ICC, FUNC
Variant
0.1 mg
12-558-C100
In stock
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.
Application
IP, WB, FC (QC tested), IHC(P), ICC, FUNC
Application details
Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: recommended dilution: 1 μg/ml.
Reactivity
Rabbit, Cat, Human
Immunogen
MOLT-4 (human T-ALL cell line)
Other names
S5.7, CVID6, TAPA1, TSPAN28
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
12-558_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.
12-558_FC_Histogram
Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81.
PubMed
Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39.
PubMed
Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7.
PubMed
Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401.
PubMed
Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149.
PubMed
Fritzsching B, Schwer B, Kartenbeck J, Pedal A, Horejsi V, Ott M: Release and intercellular transfer of cell surface CD81 via microparticles. J Immunol. 2002 Nov 15;169(10):5531-7.
PubMed
Variant
0.1 mg
12-558-C100
In stock
150.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD
Contact distributor
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