Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD81 Purified Low Endotoxin

Anti-Hu CD81 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD81

Clone

M38

Format

Purified Low Endotoxin

Reactivity

Rabbit, Cat, Human

Application

IP, WB, FC (QC tested), IHC-P, ICC, FUNC

Variant

0.1 mg

12-558-C100

In stock

165.00 USD

Variant

0.1 mg

12-558-C100

In stock

165.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody M38 reacts with an extracellular epitope of CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Application

IP, WB, FC (QC tested), IHC-P, ICC, FUNC

Application details

Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: recommended dilution: 1 μg/ml.
Western blotting: recommended dilution: 1-2 μg/ml; positive control: Jurkat cells, non-reducing conditions.

Reactivity

Rabbit, Cat, Human

Immunogen

MOLT-4 (human T-ALL cell line)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

S5.7, CVID6, TAPA1, TSPAN28

Antigen description

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells, e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Entrez Gene ID 975

UniProt ID P60033

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Western blotting analysis of human CD81 using mouse monoclonal antibody M38 on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.

Product specific references:

Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81.
PubMed

Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39.
PubMed

Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7.
PubMed

Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401.
PubMed

Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149.
PubMed

Fritzsching B, Schwer B, Kartenbeck J, Pedal A, Horejsi V, Ott M: Release and intercellular transfer of cell surface CD81 via microparticles. J Immunol. 2002 Nov 15;169(10):5531-7.
PubMed