Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD75 FITC

Anti-Hu CD75 FITC

Regulatory status

RUO

Antigen

CD75

Clone

LN1

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-922-T100

In stock

198.00 USD

Variant

100 tests

1F-922-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM kappa

Specificity

The mouse monoclonal antibody LN1 recognizes CD75, a lactosamine structure present mainly on the surface of B cell types.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Stimulated human PBL

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

lactosamine

Antigen description

CD75 is a lactosamine structure, which is present mainly on the surface of germinal center B cells. With lower level it is present on other mature B cells, and it is downregulated during differentiation to plasma cells. It is a marker which differentiates between malignant B cell types.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD75 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Fromm JR, Thomas A, Wood BL: Characterization and purification of neoplastic cells of nodular lymphocyte predominant hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol. 2017 Feb;187(2):304-317.
PubMed

Epstein AL, Marder RJ, Winter JN, Fox RI: Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028-36.
PubMed

Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66.
PubMed