Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD74 PE

Anti-Hu CD74 PE

Regulatory status

RUO

Antigen

CD74

Clone

LN2

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-500-T100

In stock

242.00 USD

Variant

100 tests

1P-500-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody LN2 recognizes an extracellular epitope of CD74 (the MHC II-associated invariant chain, Ii), a type II transmembrane protein expressed in antigen-presenting cells, as well as e.g. on activated neoplastic cells in T cell lymphomas, in lymph node germinal center, mantle zone B cells, histiocytes, interdigitating reticulum cells, Langerhans cells, thymic dendritic cells and peripheral blood B lymphocytes.

Workshop

HLDA IV: WS Code B 129; HLDA V: WS Code B CD74.3

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

SU-DHL-4 lymphoma cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

p33, DHLAG, HLADG, Ia-gamma

Antigen description

CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.

Entrez Gene ID 972

UniProt ID P04233

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD19 (4G7) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD74 positive B cells (red-filled) from CD74 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD74 (LN2) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

General references:

Hussain A, Wesley C, Khalid M, Chaudhry A, Jameel S: Human immunodeficiency virus type 1 Vpu protein interacts with CD74 and modulates major histocompatibility complex class II presentation. J Virol. 2008 Jan;82(2):893-902.
PubMed

Gore Y, Starlets D, Maharshak N, Becker-Herman S, Kaneyuki U, Leng L, Bucala R, Shachar I: Macrophage migration inhibitory factor induces B cell survival by activation of a CD74-CD44 receptor complex. J Biol Chem. 2008 Feb 1;283(5):2784-92.
PubMed

Lantner F, Starlets D, Gore Y, Flaishon L, Yamit-Hezi A, Dikstein R, Leng L, Bucala R, Machluf Y, Oren M, Shachar I: CD74 induces TAp63 expression leading to B-cell survival. Blood. 2007 Dec 15;110(13):4303-11.
PubMed

Stein R, Mattes MJ, Cardillo TM, Hansen HJ, Chang CH, Burton J, Govindan S, Goldenberg DM: CD74: a new candidate target for the immunotherapy of B-cell neoplasms. Clin Cancer Res. 2007 Sep 15;13(18 Pt 2):5556s-5563s.
PubMed

Starlets D, Gore Y, Binsky I, Haran M, Harpaz N, Shvidel L, Becker-Herman S, Berrebi A, Shachar I: Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival. Blood. 2006 Jun 15;107(12):4807-16.
PubMed

Product specific references:

Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, Goldenberg DM: CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004 Oct 1;10(19):6606-11.
PubMed

Badve S, Deshpande C, Hua Z, Lögdberg L: Expression of invariant chain (CD 74) and major histocompatibility complex (MHC) class II antigens in the human fetus. J Histochem Cytochem. 2002 Apr;50(4):473-82.
PubMed

Leng L, Metz CN, Fang Y, Xu J, Donnelly S, Baugh J, Delohery T, Chen Y, Mitchell RA, Bucala R: MIF signal transduction initiated by binding to CD74. J Exp Med. 2003 Jun 2;197(11):1467-76.
PubMed

Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Perez MT, Cabello-Inchausti B, Castellano-Sanchez A, Kottiech S, Davila E, Willis I, Barkin J: Primary gastroesophageal-ileal hodgkin lymphoma. Arch Pathol Lab Med. 2002 Dec;126(12):1534-7.
PubMed

Freeman GJ, Cardoso AA, Boussiotis VA, Anumanthan A, Groves RW, Kupper TS, Clark EA, Nadler LM: The BB1 monoclonal antibody recognizes both cell surface CD74 (MHC class II-associated invariant chain) as well as B7-1 (CD80), resolving the question regarding a third CD28/CTLA-4 counterreceptor. J Immunol. 1998 Sep 15;161(6):2708-15.
PubMed