Anti-Hu CD72 PE

Anti-Hu CD72 PE
Regulatory status
RUO
Antigen
CD72
Clone
3F3
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-310-T100
Out of stock

25 tests
1P-310-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
Workshop
HLDA V: WS Code B CD72.5; HLDA VI: WS Code B CD72.1; HLDA VI: WS Code 6 BP 84
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Normal human lymphocytes from a lymph node.
Other names
LYB2
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1P-310 FC
Flow cytometry analysis (surface staining) of CD72 on human peripheral blood cells with anti-CD72 (3F3) PE.

General references:

Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS ONE. 2007 Sep 5;2(9):e818.
PubMed
Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17(10):1277-82.
PubMed
Kumanogoh A, Kikutani H: The CD100-CD72 interaction: a novel mechanism of immune regulation. Trends Immunol. 2001 Dec;22(12):670-6.
PubMed
Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000 Nov;13(5):621-31.
PubMed

Product specific references:

Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
PubMed
Dauby N, Kummert C, Lecomte S, Liesnard C, Delforge ML, Donner C, Marchant A: Primary Human Cytomegalovirus Infection Induces the Expansion of Virus-Specific Activated and Atypical Memory B Cells. J Infect Dis. 2014 May 1. pii: jiu255. [Epub ahead of print]
PubMed
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5;2(9):e818.
PubMed
Variant
100 tests
1P-310-T100
Out of stock

25 tests
1P-310-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock