Clinical products
Premium antibodies (GMP)
Intracellular staining
Surface staining
Hematology
Kits
Lysing solutions
Premixed antibody combinations
Single color antibodies
Immunodeficiency
COVID-19 kits
Kits
Lysing solutions
Mitogens
Premixed antibody combinations
Single color antibodies
Reproductive immunology
Kits
Lysing solutions
Premixed antibody combinations
New products
Allergy
Kits
Recombinant allergens
Allergen extracts
Other allergens
Research products
Antibodies
CD and related antigens
Blood group antigens
Premixed antibody combinations
Serum, plasma, and secreted proteins
Cytoskeleton and ECM
Isotype controls
MHC antigens
Nucleus
Other intracellular antigens
Small organic molecules
Tags and labels
Microbiology and virology
Accessory reagents
Annexin binding
DNA-binding probes
Lineage cocktail
Lysing solutions
Recombinant proteins
Recombinant allergens
Animal
Food
House Dust Mite
Mold
Pollen
Venom
Kits
New products
Custom products
OEM and Commercial Supplies
About exbio
Company
Blog
Events and Conferences
Inquiries
Technical resources
International distributors
Contact
Jobs
E-mail
*
Password
*
Remember me
Forgotten password
Log in
Register
E-mail
Send request
Log in
Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD72 Alexa Fluor<sup>®</sup> 488
Anti-Hu CD72 Alexa Fluor
®
488
Regulatory status
RUO
Antigen
CD72
Clone
3F3
Format
Alexa Fluor® 488
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
A4-310-T100
In stock
220.00 USD
25 tests
A4-310-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
Workshop
HLDA V: WS Code B CD72.5; HLDA VI: WS Code B CD72.1; HLDA VI: WS Code 6 BP 84
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Normal human lymphocytes from a lymph node.
Other names
LYB2
Preparation
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD72 (3F3) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).
Separation of human CD72 positive lymphocytes (red-filled) from CD72 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD72 (3F3) Alexa Fluor® 488 antibody (4 μl reagent / 100 μl of peripheral whole blood).
General references:
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS ONE. 2007 Sep 5;2(9):e818.
PubMed
Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H. Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment. Int Immunol. 2005 Oct;17(10):1277-82.
PubMed
Kumanogoh A, Kikutani H: The CD100-CD72 interaction: a novel mechanism of immune regulation. Trends Immunol. 2001 Dec;22(12):670-6.
PubMed
Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000 Nov;13(5):621-31.
PubMed
Product specific references:
Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61.
PubMed
Dauby N, Kummert C, Lecomte S, Liesnard C, Delforge ML, Donner C, Marchant A: Primary Human Cytomegalovirus Infection Induces the Expansion of Virus-Specific Activated and Atypical Memory B Cells. J Infect Dis. 2014 May 1. pii: jiu255. [Epub ahead of print]
PubMed
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS One. 2007 Sep 5;2(9):e818.
PubMed
Variant
100 tests
A4-310-T100
In stock
220.00 USD
25 tests
A4-310-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
Datasheet download
SDS download
CS
EN
Bulk requests
Technical support for this product
Continue to checkout
×
Zavřít