Anti-Hu CD71 Purified

Anti-Hu CD71 Purified
Regulatory status
RUO
Antigen
CD71
Clone
MEM-189
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-353-C100
In stock
143.00 USD

0.025 mg
11-353-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-353-C100
In stock
143.00 USD

0.025 mg
11-353-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-189 reacts with an extracellular epitope on CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
Workshop
HLDA VI: WS Code NL N-L025
Application details
Flow cytometry: Recommended dilution: 2 μg/ml.
Western blotting: Non-reducing conditions.
Reactivity
Human
Immunogen
KG1 human acute myelogenous leukaemia cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TfR1, TfR, TR, Trfr, T9, p90, TFRC
Antigen description
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Entrez Gene ID 7037
UniProt ID P02786
11-353 FC
Separation of K562 cells stained using anti-human CD71 (MEM-189) purified antibody (concentration in sample 4 μg/ml, GAM APC, red) from K562 cells unstained by primary antibody (GAM APC, grey) in flow cytometry analysis (surface staining).

General references:

Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36.
PubMed
Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34.
PubMed
Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
PubMed
Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79.
PubMed

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Variant
0.1 mg
11-353-C100
In stock
143.00 USD

0.025 mg
11-353-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-353-C100
In stock
143.00 USD

0.025 mg
11-353-C025
Delivery 1 week
71.50 USD

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