Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD71 Biotin

Anti-Hu CD71 Biotin

Anti-Hu CD71 Biotin
Regulatory status
RUO
Antigen
CD71
Clone
MEM-75
Format
Biotin
Reactivity
Human
Application
IP, FC (QC tested), ICC
Variant
0.1 mg
1B-235-C100
In stock
180.00 USD
0.025 mg
1B-235-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody MEM-75 reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.
Workshop
HLDA IV: WS Code A 45; HLDA V: WS Code T T-165
Application
IP, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Human
Immunogen
NALM-6 human pre-B cell line
Other names
TfR1, TfR, TR, Trfr, T9, p90, TFRC
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1B-235_FC_Profil Značení
Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD71 (MEM-75) Biotin antibody (concentration in sample 0,6 μg/ml, Streptavidin APC).
1B-235_FC_Histogram
Separation of cells stained using anti-human CD71 (MEM-75) Biotin antibody (concentration in sample 0,6 μg/ml, Streptavidin APC, red-filled) from cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (surface staining).

General references:

Graham RM, Chua AC, Herbison CE, Olynyk JK, Trinder D: Liver iron transport. World J Gastroenterol. 2007 Sep 21;13(35):4725-36.
PubMed
Graham RM, Reutens GM, Herbison CE, Delima RD, Chua AC, Olynyk JK, Trinder D: Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. J Hepatol. 2008 Feb;48(2):327-34.
PubMed
Taketani S: Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation. Tohoku J Exp Med. 2005 Apr;205(4):297-318.
PubMed
Rouault TA: How mammals acquire and distribute iron needed for oxygen-based metabolism. PLoS Biol. 2003 Dec;1(3):E79.
PubMed

Product specific references:

Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb;51(2):286-96.
PubMed
Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853.
PubMed
Variant
0.1 mg
1B-235-C100
In stock
180.00 USD
0.025 mg
1B-235-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product