Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD70 Purified

Anti-Hu CD70 Purified

Regulatory status

RUO

Antigen

CD70

Clone

Ki-24

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-154-C100

In stock

130.00 USD

0.025 mg

11-154-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-154-C100

In stock

130.00 USD

0.025 mg

11-154-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG3 kappa

Specificity

The mouse monoclonal antibody Ki-24 recognizes an extracellular epitope of CD70, an approximately 50 kDa type II transmembrane glycoprotein expressed on activated lymphocytes and some B cell leukemias.

Workshop

HLDA IV: WS Code: A109

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

human L428 cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CD27L, TNFSF7, CD27 ligand, TNLG8A

Antigen description

CD70, also known as TNFSF7 or CD27L, is a 50 kDa type II transmembrane glycoprotein of the TNF superfamily. It is expressed mainly on activated lymphocytes, including NK cells, and forms trimeric structure. CD70 plays a role in T-cell activation, proliferation and differentiation, in enhancing the generation of cytolytic T cells, and in long-term maintenance of T cell memory. It is also involved in B cell differentiation induced by activated plasmacytoid dendritic cells, which also express CD70.

Entrez Gene ID 970

UniProt ID P32970

Flow cytometry surface staining pattern of HUT-78 cells stained using anti-human CD70 (Ki-24) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Separation of HUT-78 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD70 (Ki-24) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Product specific references:

Hashimoto-Okada M, Kitawaki T, Kadowaki N, Iwata S, Morimoto C, Hori T, Uchiyama T: The CD70-CD27 interaction during the stimulation with dendritic cells promotes naive CD4(+) T cells to develop into T cells producing a broad array of immunostimulatory cytokines in humans. Int Immunol. 2009 Aug;21(8):891-904.
PubMed

Israel BF, Pickles RJ, Segal DM, Gerard RD, Kenney SC: Enhancement of adenovirus vector entry into CD70-positive B-cell lines by using a bispecific CD70-adenovirus fiber antibody. J Virol. 2001 Jun;75(11):5215-21.
PubMed

Trebing J, El-Mesery M, Schäfer V, Weisenberger D, Siegmund D, Silence K, Wajant H. CD70-restricted specific activation of TRAILR1 or TRAILR2 using scFv-targeted TRAIL mutants. Cell Death Dis. 2014 Jan 30;5:e1035.
PubMed

Kaufmann Y, Amariglio N, Rosenthal E, Hirsch YJ, Many A, Rechavi G: Proliferation response of leukemic cells to CD70 ligation oscillates with recurrent remission and relapse in a low-grade lymphoma. J Immunol. 2005 Nov 15;175(10):6940-7.
PubMed