Anti-Hu CD70 APC

Anti-Hu CD70 APC
Regulatory status
RUO
Antigen
CD70
Clone
Ki-24
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
1A-154-T100
In stock
242.00 USD
Variant
100 tests
1A-154-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG3 kappa
Specificity
The mouse monoclonal antibody Ki-24 recognizes an extracellular epitope of CD70, an approximately 50 kDa type II transmembrane glycoprotein expressed on activated lymphocytes and some B cell leukemias.
Workshop
HLDA IV: WS Code: A109
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human L428 cell line
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CD27L, TNFSF7, CD27 ligand, TNLG8A
Antigen description
CD70, also known as TNFSF7 or CD27L, is a 50 kDa type II transmembrane glycoprotein of the TNF superfamily. It is expressed mainly on activated lymphocytes, including NK cells, and forms trimeric structure. CD70 plays a role in T-cell activation, proliferation and differentiation, in enhancing the generation of cytolytic T cells, and in long-term maintenance of T cell memory. It is also involved in B cell differentiation induced by activated plasmacytoid dendritic cells, which also express CD70.
Entrez Gene ID 970
UniProt ID P32970
1A-154_FC_Histogram
Separation of HUT-78 cells stained using anti-human CD70 (Ki-24) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 5 μg/ml, same as CD70 APC concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Hashimoto-Okada M, Kitawaki T, Kadowaki N, Iwata S, Morimoto C, Hori T, Uchiyama T: The CD70-CD27 interaction during the stimulation with dendritic cells promotes naive CD4(+) T cells to develop into T cells producing a broad array of immunostimulatory cytokines in humans. Int Immunol. 2009 Aug;21(8):891-904.
PubMed
Israel BF, Pickles RJ, Segal DM, Gerard RD, Kenney SC: Enhancement of adenovirus vector entry into CD70-positive B-cell lines by using a bispecific CD70-adenovirus fiber antibody. J Virol. 2001 Jun;75(11):5215-21.
PubMed
Trebing J, El-Mesery M, Schäfer V, Weisenberger D, Siegmund D, Silence K, Wajant H. CD70-restricted specific activation of TRAILR1 or TRAILR2 using scFv-targeted TRAIL mutants. Cell Death Dis. 2014 Jan 30;5:e1035.
PubMed
Kaufmann Y, Amariglio N, Rosenthal E, Hirsch YJ, Many A, Rechavi G: Proliferation response of leukemic cells to CD70 ligation oscillates with recurrent remission and relapse in a low-grade lymphoma. J Immunol. 2005 Nov 15;175(10):6940-7.
PubMed
Variant
100 tests
1A-154-T100
In stock
242.00 USD
Variant
100 tests
1A-154-T100
In stock
242.00 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
220.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
66.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
55.00 USD