Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD7 Purified

Anti-Hu CD7 Purified

Regulatory status

RUO

Antigen

CD7

Clone

MEM-186

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-206-C100

In stock

143.00 USD

0.025 mg

11-206-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-206-C100

In stock

143.00 USD

0.025 mg

11-206-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-186 reacts with an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.

Workshop

HLDA VI: WS Code T 6T-015

Application

IP, WB, FC (QC tested), IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: spleen.
Flow cytometry: Recommended dilution: 2 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Human

Immunogen

Human acute myelogenous leukaemia cell line KG-1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GP40, TLA, TP41, LEU-9

Antigen description

CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.

Entrez Gene ID 924

UniProt ID P09564

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD7 (MEM-186) purified antibody (concentration in sample 0,33 μg/ml, GAM APC).

Separation of human CD7 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD7 (MEM-186) purified antibody (concentration in sample 0,33 μg/ml, GAM APC).

General references:

Nishimura M, Takanashi M, Okazaki H, Satake M, Nakajima K: Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. Endothelium. 2006 Jul-Aug;13(4):287-92.
PubMed

Chang H, Yeung J, Brandwein J, Yi QL: CD7 expression predicts poor disease free survival and post-remission survival in patients with acute myeloid leukemia and normal karyotype. Leuk Res. 2007 Feb;31(2):157-62. Epub 2006 Jul 11.
PubMed

Lam GK, Liao HX, Xue Y, Alam SM, Scearce RM, Kaufman RE, Sempowski GD, Haynes BF. Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 2005 Jan;25(1):41-9.
PubMed

Alaibac M, Pigozzi B, Belloni-Fortina A, Michelotto A, Saponeri A, Peserico A. CD7 expression in reactive and malignant human skin T-lymphocytes. Anticancer Res. 2003 May-Jun;23(3B):2707-10.
PubMed