Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD7 Alexa Fluor® 700

Anti-Hu CD7 Alexa Fluor® 700

Anti-Hu CD7 Alexa Fluor® 700
Regulatory status
RUO
Antigen
CD7
Clone
124-1D1
Format
Alexa Fluor® 700
Reactivity
Human
Application
FC
Excitation laser
red (633 nm)
Variant
100 tests
A7-183-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 124-1D1 recognizes an extracellular epitope of CD7, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.
Workshop
HLDA IV: WS Code T155
Application
FC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
not available
Preparation
The purified antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
GP40, TLA, TP41, LEU-9
Antigen description
CD7, also known as gp40, is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.
Entrez Gene ID 924
UniProt ID P09564
A7-183_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD7 (124-1D1) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).
A7-183_FC_Histogram
Separation of human CD7 positive lymphocytes (red-filled) from CD7 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD7 (124-1D1) Alexa Fluor® 700 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Nishimura M, Takanashi M, Okazaki H, Satake M, Nakajima K: Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. Endothelium. 2006 Jul-Aug;13(4):287-92.
PubMed
Chang H, Yeung J, Brandwein J, Yi QL: CD7 expression predicts poor disease free survival and post-remission survival in patients with acute myeloid leukemia and normal karyotype. Leuk Res. 2007 Feb;31(2):157-62. Epub 2006 Jul 11.
PubMed
Lam GK, Liao HX, Xue Y, Alam SM, Scearce RM, Kaufman RE, Sempowski GD, Haynes BF. Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma. J Clin Immunol. 2005 Jan;25(1):41-9.
PubMed
Alaibac M, Pigozzi B, Belloni-Fortina A, Michelotto A, Saponeri A, Peserico A. CD7 expression in reactive and malignant human skin T-lymphocytes. Anticancer Res. 2003 May-Jun;23(3B):2707-10.
PubMed

Product specific references:

Pelegrí C, Castell M, Serra M, Rabanal M, Rodríguez-Palmero M, Castellote C, Franch A: Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells. Clin Exp Immunol. 2001 Sep;125(3):470-7.
PubMed
Vicario M, Amat C, Rivero M, Moretó M, Pelegrí C: Dietary glutamine affects mucosal functions in rats with mild DSS-induced colitis. J Nutr. 2007 Aug;137(8):1931-7.
PubMed
Preston SG, Majtán J, Kouremenou C, Rysnik O, Burger LF, Cabezas Cruz A, Chiong Guzman M, Nunn MA, Paesen GC, Nuttall PA, Austyn JM: Novel immunomodulators from hard ticks selectively reprogramme human dendritic cell responses. PLoS Pathog. 2013;9(6):e1003450.
PubMed
van Dongen JJ, Lhermitte L, Böttcher S, Almeida J, van der Velden VH, Flores-Montero J, Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepański T, Kalina T, de Tute R, Brüggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin-Ayuso M, Hrusak O, Vidriales MB, Orfao A; EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708): EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012 Sep;26(9):1908-75. doi: 10.1038/leu.2012.120.
PubMed
Variant
100 tests
A7-183-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product