Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD68 PE

Anti-Hu CD68 PE

Regulatory status

RUO

Antigen

CD68

Clone

Y1/82A

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-749-T100

In stock

242.00 USD

25 tests

1P-749-T025

In stock

121.00 USD

Variant

100 tests

1P-749-T100

In stock

242.00 USD

25 tests

1P-749-T025

In stock

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody Y1/82A recognizes CD68 (LAMP4), a 110 kDa glycoprotein expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells.

Workshop

HLDA IV, HLDA VI

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Reactivity

Human

Immunogen

Lysosomal contents of lung macrophages

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GP110, LAMP4, SCARD1

Antigen description

CD68 (also known as LAMP4 or SCARD1) is a 110 kDa type I transmembrane glycoprotein of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and the scavenger receptor family. Although CD68 primarily localizes to lysosomes and endosomes, its fraction circulates to the cell surface. By the heavily glycosylated extracellular domain CD68 binds to tissue- and organ-specific lectins or selectins. It is expressed mainly in cytoplasmic granules of monocytes/macrophages, granulocytes, and dendritic cells, but also e.g. in a proportion of epithelial tumours (diagnosis of poorly differentiated neoplasms).

Entrez Gene ID 968

UniProt ID P34810

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD68 (Y1/82A) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human monocytes (red-filled) from CD68 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD68 (Y1/82A) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 Apr;46(4):334-6.
PubMed

Ulanova M, Tarkowski A, Hahn-Zoric M, Hanson LA: The Common vaccine adjuvant aluminum hydroxide up-regulates accessory properties of human monocytes via an interleukin-4-dependent mechanism. Infect Immun. 2001 Feb;69(2):1151-9.
PubMed

Mack CL, Tucker RM, Sokol RJ, Karrer FM, Kotzin BL, Whitington PF, Miller SD: Biliary atresia is associated with CD4+ Th1 cell-mediated portal tract inflammation. Pediatr Res. 2004 Jul;56(1):79-87.
PubMed

I A Doussis, K C Gatter, and D Y Mason: CD68 reactivity of non-macrophage derived tumours in cytological specimens. J Clin Pathol. 1993 April; 46(4): 334–336.
PubMed

Yamagami S, Yokoo S, Amano S, Ebihara N: Characterization of bone marrow derived cells in the substantia propria of the human conjunctiva. Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4476-81.
PubMed

Holness CL, Simmons DL: Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood. 1993 Mar 15;81(6):1607-13.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed