Anti-Hu CD66e Purified

Anti-Hu CD66e Purified
Regulatory status
RUO
Antigen
CD66e
Clone
CB30
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-479-C100
In stock
143.00 USD

0.025 mg
11-479-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-479-C100
In stock
143.00 USD

0.025 mg
11-479-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody CB30 recognizes CD66e (CEA; 180-200 kDa), an extracellular cell surface-bound carcinoembryonic antigen mainly expressed on epithelial cells.
Application details
Immunohistochemistry (paraffin sections): Staining technique: standard ABC technique (DAB+), recommended dilution: 10 μg/ml (1:100), positive tissue: adenocarcinoma of colon, pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature.
Reactivity
Human
Immunogen
Human carcinoembryonic antigen (CEA; CEACAM5)
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CEACAM5, CEA
Antigen description
The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion. Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.
Entrez Gene ID 1048
UniProt ID P06731
11-479_IHC
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30). Primary antibody dilution: 10 μg/ml.
11-479_IHC_2
Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30). Primary antibody dilution: 10 μg/ml.

General references:

Oikawa S, Nakazato H, Kosaki G: Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence. Biochem Biophys Res Commun. 1987 Jan 30;142(2):511-8.
PubMed
Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal. 1991;5(5):344-66. Review.
PubMed
Gangopadhyay A, Bajenova O, Kelly TM, Thomas P: Carcinoembryonic antigen induces cytokine expression in Kuppfer cells: implications for hepatic metastasis from colorectal cancer. Cancer Res. 1996 Oct 15;56(20):4805-10.
PubMed
Chen CJ, Li LJ, Maruya A, Shively JE: In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment. Cancer Res. 1995 Sep 1;55(17):3873-82.
PubMed

Product specific references:

Koga S, Oshima Y, Honkura N, Iimura T, Kameda K, Sato K, Yoshida M, Yamamoto Y, Watanabe Y, Hikita A, Imamura T: In vivo subcellular imaging of tumors in mouse models using a fluorophore-conjugated anti-carcinoembryonic antigen antibody in two-photon excitation microscopy. Cancer Sci. 2014 Oct;105(10):1299-306.
PubMed
Wang L, Ma N, Okamoto S, Amaishi Y, Sato E, Seo N, Mineno J, Takesako K, Kato T, Shiku H: Efficient tumor regression by adoptively transferred CEA-specific CAR-T cells associated with symptoms of mild cytokine release syndrome. Oncoimmunology. 2016 Jul 25;5(9):e1211218.
PubMed
Variant
0.1 mg
11-479-C100
In stock
143.00 USD

0.025 mg
11-479-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-479-C100
In stock
143.00 USD

0.025 mg
11-479-C025
Delivery 1 week
71.50 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
220.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
66.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
55.00 USD
-134%
CloneMOPC-21
Reg. statusRUO
0.1 mg
115.50 USD